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Different previus inoculum, temperature and incubation time in the production of aflatoxin B1 in rice

The production of aflatoxin in rice by three isolates of Aspergillus flavus was investigated for different culture conditions (temperature and incubation time) and previous inoculum (YES- Yeast Extrat Sucrose and CYA- Czapeck Yeast Extrat). All strains withdrawn from the previous inoculum medium YES and inoculated in the rice in temperature of 25°C/18 days and 20°C/14 days, produced aflatoxin B1. The medium CYA had lower performance since the three strains tested did not produce aflatoxin B1 in the combination 20°C/14 days. At 25°C/11 days of incubation time aflatoxin was not detectable.

Aspergillus flavus; CYA; YES; incubation time; temperature; rice


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