The aim of the present research was to compare the efficiency of coconut water and Tris extenders on canine semen freezing. The semen from 5 German Shepherd dogs was collected by digital manipulation. The spermatic fraction of different dogs was evaluated about its macro and microscopic parameters and mixed in a pool. It was extended in coconut water or Tris, added of egg yolk (10 or 20%) and glycerol (4, 6 or 8%). Then, it was frozen by the method of NUNES et al. (1997) and thawed at 37ºC after one week. A Tris extender superiority was shown at the conservation of vigor (2.3 ± 0.8), total (14.8 ± 5.1%) and secondary (14.4 ± 5.5%) spermatic defects in the protocols with 20% of egg yolk and 6% of glycerol. In all other protocols, there were no significant differences for the observed parameters. These results suggest a superiority of Tris extender over the coconut water extender on canine semen freeze, in the use of them added of 20% of egg yolk and 6% of glycerol.
semen; dog; freeze; coconut water; Tris; glycerol; egg yolk
