Occurrence and richness of arbuscular mycorrizal fungi in vineyards with grapevine decline and dieback symptoms

Bezerra, Aelton dos Santos; Betancur-Agudelo, Marcelo; Meyer, Edenilson; Kemmelmeier, Karl; Stürmer, Sidney Luiz; Soares, Cláudio Roberto Fonsêca Sousa; Lovato, Paulo Emilio

doi:10.1590/0103-8478cr20210011

Acessibilidade / Reportar erro

Brasil
SciELO.org - Rede SciELO
Blog SciELO em Perspectiva

Abrir menu

Brasil

Español English

Ciência Rural

Submissão de manuscritos
Sobre o periódico
Corpo Editorial
Instruções aos autores
Contato

sumário « anterior atual seguinte »

Resumo (Inglês)
Resumo (Português)

Texto (Inglês)

Download PDF (Inglês)

Compartilhe

Compartilhe
E-mail
Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley

Sumário

Compartilhe
E-mail
Facebook
Twitter
Google+
LinkedIn
Reddit
StambleUpon
CiteULike
Mendeley

Resumo (Inglês)
Resumo (Português)

Texto (Inglês)

Download PDF (Inglês)

CROP PROTECTION • Cienc. Rural 52 (3) • 2022 • https://doi.org/10.1590/0103-8478cr20210011 copy

Occurrence and richness of arbuscular mycorrizal fungi in vineyards with grapevine decline and dieback symptoms

Ocorrência e riqueza de fungos micorrízicos arbusculares em vinhedos com sintomas de declínio e morte da videira

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

This research identified arbuscular mycorrhizal fungi (AMF) in rhizosphere soil of grapevines with Grapevine Death and Decline symptoms (GDD) or asymptomatic healthy (H) plants, and characterized the relationship of AMF communities with soil chemical attributes. The AMF spore number ranged from 287 to 432 spores 50 cm^-3 in soil with GDD plants, and from 357 to 464 spores 50 cm^-3 in H plants, with no differences among vineyards or between GDD and H plants within each vineyard. We detected 42 species and 17 genera, and most taxa belonged to Acaulosporaceae or Glomeraceae. Claroideoglomus etunicatum, Funneliformis mosseae, and Archaeospora trappei were the most frequent species in all vineyards. Soil chemical attributes were not determinant for the occurrence of most fungal species; although, Entrophospora infrequens, Diversispora sp1 and Diversispora sp2 were associated with a vineyard having high soil copper. Vineyards harbor highly diverse AMF communities, which are determined by location.

Key words:
community structure; glomeromycota; soil factors; Vitis

Authorship

Aelton dos Santos Bezerra

Departamento de Engenharia Rural, Universidade Federal de Santa Catarina (UFSC), 88040-900, Florianópolis, SC, Brasil..Universidade Federal de Santa Catarina (UFSC)BrazilFlorianópolis, SC, BrazilDepartamento de Engenharia Rural, Universidade Federal de Santa Catarina (UFSC), 88040-900, Florianópolis, SC, Brasil..

http://orcid.org/0000-0002-2103-5080

Marcelo Betancur-Agudelo

http://orcid.org/0000-0001-5681-7800

Edenilson Meyer

http://orcid.org/0000-0001-6094-0641

Karl Kemmelmeier

Departamento de Ciências Naturais, Universidade Regional de Blumenau (FURB), Blumenau, SC, Brasil.Universidade Regional de Blumenau (FURB)BrazilBlumenau, SC, BrazilDepartamento de Ciências Naturais, Universidade Regional de Blumenau (FURB), Blumenau, SC, Brasil.

http://orcid.org/0000-0001-8298-0900

Sidney Luiz Stürmer

http://orcid.org/0000-0003-3213-1841

Cláudio Roberto Fonsêca Sousa Soares

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina (UFSC)BrazilFlorianópolis, SC, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brasil.

http://orcid.org/0000-0002-5443-6614

Paulo Emilio Lovato ^*E-mail: paulo.lovato@ufsc.br. *Corresponding author

http://orcid.org/0000-0001-5828-696X

E-mail: paulo.lovato@ufsc.br. ^*Corresponding author

DECLARATION OF CONFLICT OF INTEREST

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS’ CONTRIBUTIONS

All authors contributed equally for the conception and writing of the manuscript. All authors critically revised the manuscript and approved of the final version.

Editor:

Leandro Souza da Silva

SCIMAGO INSTITUTIONS RANKINGS

Figures | Tables

Figures (4)
Tables (5)

Thumbnail

Figure 1
AMF spores 50 cm-3 of soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms. V1 - V4: vineyard; GDD: plants with GDD symptoms; H: asymptomatic plants. Bars represent standard error of the mean.

Thumbnail

Figure 2
Venn diagram of the presence of AMF species in the soil around grapevines with Grapevine Decline and Dieback symptoms (GDD) or asymptomatic (H) in four vineyards (V1 to V4).

Thumbnail

Figure 3
Principal coordinate analysis (PCoA) using a similarity coefficient of Jaccard for the presence-absence data of arbuscular mycorrhizal fungal species in the soil around grapevines with (GDD) or without (H) Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Thumbnail

Figure 4
Distance-based redundancy analysis (dbRDA) between soil chemical attributes and occurrence of arbuscular mycorrhizal fungi (AMF) species in the soil around grapevines with (D) or without (H) Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Thumbnail

Table 1
General information on four vineyards in the Rio do Peixe Valley, Santa Catarina,, Brazil.

Thumbnail

Table 2
Soil chemical attributes in four vineyards in the Rio do Peixe Valley, Santa Catarina, Brazil.

Thumbnail

Table 3
Occurrence frequency and global frequency (F%) of arbuscular mycorrhizal fungus species in soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Thumbnail

Table 4
Permanova using Jaccard similarity coefficient for presence-absence data of AMF species in soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Thumbnail

Table 5
Eigenvalues explained variability and dbRDA analysis coordinates for chemical attributes of soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms, in four vineyards (V1 to V4).

Figure 1 AMF spores 50 cm-3 of soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms. V1 - V4: vineyard; GDD: plants with GDD symptoms; H: asymptomatic plants. Bars represent standard error of the mean.

Figure 2 Venn diagram of the presence of AMF species in the soil around grapevines with Grapevine Decline and Dieback symptoms (GDD) or asymptomatic (H) in four vineyards (V1 to V4).

Figure 3 Principal coordinate analysis (PCoA) using a similarity coefficient of Jaccard for the presence-absence data of arbuscular mycorrhizal fungal species in the soil around grapevines with (GDD) or without (H) Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Figure 4 Distance-based redundancy analysis (dbRDA) between soil chemical attributes and occurrence of arbuscular mycorrhizal fungi (AMF) species in the soil around grapevines with (D) or without (H) Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Table 1 General information on four vineyards in the Rio do Peixe Valley, Santa Catarina,, Brazil.

	----------------------------------------------Municipality / Vineyards Identification-----------------------------------------------
	Videira (V1)	Pinheiro Preto (V2)	Tangará (V3)	Videira (V4)
Rootstock/graft	VR 043-43/Bordô	VR 043-43/ Isabel Precoce	Paulsen-1103/ Isabel Precoce	Paulsen1103/Chardonnay
Age (years)	>8	>8	>30	>10
Soil classification	Nitossol	Nitossol	Nitossol	Nitossol
Area (ha)	0.6	0.5	2.0	0.7
Altitude (m)	719	709	641	719
Coordinates	S 27°03'53.3'' W 51°09'54.8''	S 27°04'03.1'' W51°10'42.9''	S 27°05'30.2'' W51°12'59.2''	S 27°01'57.1'' W51°08'02.7''

Table 2 Soil chemical attributes in four vineyards in the Rio do Peixe Valley, Santa Catarina, Brazil.

	V1	V2	V3	V4
pH in H₂O	5.7	6.1	6.5	5.1
H+Al (mmolc dm^-3)	55	42	28	69
K(mg dm^-3)	427	486	185	158
P Melich (mg dm^-3)	34	154	90	52
P resin (mg dm^-3)	38	130	53	46
Al (mmol_c dm^-3)	0.31	0.04	0.00	1.10
Ca (mmol_c dm^-3)	86	172	155	85
Mg (mmol_c dm^-3)	37	45	41	30
Cu Melich (mg dm^-3)	9.0	23	65	10
CEC (mmol_c dm^-3)	189	272	229	188
Clay (g kg^-1)	490	360	470	650
Organic matter (g kg^-1)	34	31	28	36

Table 3 Occurrence frequency and global frequency (F%) of arbuscular mycorrhizal fungus species in soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Species in field samples or trap culture	--Plants with GDD symptoms--				-------Asymptomatic plants------
Family / Species	V1	V2	V3	V4	V1	V2	V3	V4	F%
Family Acaulosporaceae
Acaulospora alpina Oehl, Sýkorová and Sieverd	-	-	-	-	-	-	R	-	1.8
Acaulospora foveata Trappe & Janos	-	-	-	-	-	R	-	-	1.8
Acaulospora mellea Spain and N.C. Schenck	VC	C	R	D	VC	R	VC	R	46.4
Acaulospora morrowiae cf. Spain and N.C. Schenck	D	R	-	R	C	R	R	R	25.0
Acaulospora reducta Oehl, B.T. Goto and C.M.R. Pereira	-	R	-	-	-	-	R	-	3.6
Acaulospora scrobiculata Trappe	R	R	VC	R	C	-	R	R	28.6
Acaulospora tuberculata Janos and Trappe	R	-	-	-	-	-	-	-	3.6
Acaulospora walkericf. Kramad. and Hedger	-	-	-	-	-	-	R	R	3.6
Family Ambisporaceae
Ambispora leptoticha(N.C. Schenck and G.S. Sm.) R.J. Bills & J.B. Morton	VC	-	-	-	-	R	-	-	8.9
Ambispora reticulata Oehl and Sieverd	-	-	-	R	-	R	-	VC	12.5
Family Archaeosporaceae
Archaeospora trappei (R.N. Ames and Linderman) J.B. Morton and D. Redecker	D	D	D	D	VC	D	D	VC	83.9
Family Claroideoglomeraceae
Claroideoglomus claroideum (N.C. Schenck and G.S. Sm.) C. Walker and A. Schüßler	R	-	VC	-	-	VC	R	-	17.9
Claroideoglomus etunicatum (W.N. Becker and Gerd.) C. Walker and A. Schüßler	D	D	VC	D	D	VC	D	D	92.9
Family Diversisporaceae
Diversispora sp1	D	D	VC	VC	D	VC	C	D	75.0
Diversispora sp2	-	R	D	D	R	-	D	VC	48.2
Diversispora sp3	-	-	R	R	-	-	D	-	21.4
Family Gigasporaceae
Cetraspora pellucida (T.H. Nicolson and N.C. Schenck) Oehl, F.A. Souza and Sieverd	C	D	D	C	VC	R	D	R	57.1
Gigaspora margarita cf. W.N. Becker and I.R. Hall	R	R	-	D	R	-	R	D	33.9
Dentiscutata heterogama (T.H. Nicolson and Gerd.) Sieverd., F.A. Souza and Oehl	-	-	-	R	R	-	R	R	5.4
Family Glomeraceae
Dominikia aureacf. (Oehl and Sieverd.) Błaszk., Chwat, G.A. Silva and Oehl	R	R	R	-	-	R	-	-	8.9
Funneliformis mosseae(T.H. Nicolson and Gerd.) C. Walker and A. Schüßler	D	D	D	D	VC	D	D	D	89.3
Glomus microaggregatum Koske, Gemma and P.D. Olexia	C	R	-	C	-	-	-	VC	21.4
Glomus ambisporum cf. G.S. Sm. and N.C. Schenck	VC	VC	VC	D	VC	D	VC	C	67.9
Glomus spinuliferum Sieverd. and Oehl	-	-	-	R	R	-	-	-	3.6
Oehlia diaphana(J.B. Morton and C. Walker) Blaszk., Kozlowska & Dalpé	R	-	-	-	-	-	-	-	1.8
Glomus microcarpum Tul. & C. Tul.	R	-	R	R	-	-	R	-	8.9
Rhizophagus clarus (T.H. Nicolson and N.C. Schenck) C. Walker and A. Schüßler	-	-	-	C	-	-	-	R	8.9
Rhizophagus fasciculatus (Thaxt.) C. Walker and A. Schüßler	-	-	-	-	R	-	-	-	1.8
Septoglomus viscosum (T.H. Nicolson) C. Walker, D. Redecker, Stille and A. Schüßler	R	VC	D	VC	C	VC	VC	D	62.5
Septoglomus constrictum (Trappe) Sieverd., G.A. Silva and Oehl	R	-	-	-	-	-	-	-	1.8
Sclerocystis sinuosaGerd. and B.K. Bakshi	C	C	VC	R	VC	R	VC	-	39.3
Sclerocystis coremioidesBerk. and Broome	-	-	-	-	-	-	R	R	5.4
Glomus sp1	D	VC	C	D	D	VC	C	D	71.4
Glomus sp2	VC	C	R	D	VC	VC	R	D	57.1
Glomus sp3^*	-	-	-	-	-	-	-	R	1.8
Glomus sp4^*	-	-	-	-	-	-	-	R	1.8
Rhizophagus sp1	C	R	R	R	C	-	R	R	21.4
Rhizophagus sp2	R	-	R	R	R	R	R	-	16.1
Sclerocystis sp1^*	-	-	-	-	R	-	-	-	1.8
Incertae sedis
Entrophospora infrequens (I.R. Hall) R.N. Ames and R.W. Schneid	C	-	D	D	VC	R	D	VC	62.5
Family Paraglomeraceae
Paraglomus occultum (C. Walker) J.B. Morton and D. Redecker	D	R	C	R	C	R	-	R	33.9
Paraglomus brasilianum (Spain and J. Miranda) J.B. Morton and D. Redecker	-	-	R	-	R	-	-	-	3.6

D = dominant (85% ≤ FO ≤ 100%), VC = very common (50% ≤ FO < 85%), C = common (30% ≤ FO < 50%), R = rare (FO < 30%), ^*recovered exclusively from trap cultures.

Table 4 Permanova using Jaccard similarity coefficient for presence-absence data of AMF species in soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms in four vineyards (V1 to V4).

Source	Sum of squares	Degrees of freedom	Medium square	F	p
Vineyards	2.9664	3	0.9888	6.9637	0.0001
GDD or Asymptomatic	0.0969	1	0.09699	0.6831	0.7757
Interaction	0.7634	3	0.25447	1.7921	0.0039
Residual	6.8156	48	0.14199
Total	10.642	55

Table 5 Eigenvalues explained variability and dbRDA analysis coordinates for chemical attributes of soil around grapevines with or without Grapevine Decline and Dieback (GDD) symptoms, in four vineyards (V1 to V4).

Main Components	-------------------------Coordinates-----------------------
	CAP1	CAP2
Eigenvalues ^†	1.548	0.868
Explained variability (%)	40.43	22.67
Explained accumulated variability (%)	40.43	63.10
------------------------------------------------------------------------------Variable-----------------------------------------------------------------------------
P.r	-0.03	-0.62^*
pH	0.70^*	-0.43
K	-0.37	-0.84^*
M.O	-0.69^*	0.45
Ca	0.54^*	-0.64^*
Mg	0.19	-0.64^*
Cu	0.91^*	-0.10

^†Components with eigenvalues > 1 explain > 10% of the total variance considered.
^* Variables with significant projections on the main components (Value > 0.50) (SANTOS et al. 2016).

How to cite

copy

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Occurrence and richness of arbuscular mycorrizal fungi in vineyards with grapevine decline and dieback symptoms

PlumX

Mensagem

[1] E-mail: paulo.lovato@ufsc.br. ^*Corresponding author