Analysis on genetic diversity of 51 Grape germplasm resources

Yue, Qingchun; Zhang, Chenfei; Wang, Qinghao; Wang, Wenjing; Wang, Jinyang; Wu, Yueyan

doi:10.1590/0103-8478cr20190247

ABSTRACT:

The objective of this study is to research the genetic diversity of the ‘ Zuijinxiang ’ grape and its mutant breeding F1 plants, we screened the excellent mutant plants with potential breeding value. 50 mutated single plants obtained from ¹³⁷Cs-γ irradiated ‘Zuijinxiang’ grape seeds were used as research objects, and SCoT molecular marker technology was used for genetic diversity and variation analysis, and clustering research was carried out. The results showed that: (1) 36 SCoT primers produced abundant polymorphisms, and the amplification results showed obvious bright bands, and the amplification efficiency and polymorphism rate were 100%. (2) A total of 221 bands were amplified by 36 primers, of which 175 were rich in polymorphism, the average polymorphic percentage was 80.3%, and the average genetic similarity coefficient was 0.916. (3) The number of observed alleles (Na) ranged from 4 to 8, with an average of 6.1389; the number of effective alleles (Ne) ranged from 1.2772 to 5.6322 with an average of 3.5968; the desired heterozygosity (He) The range is from 0.2192 to 0.8344, the average is 0.6965; the observed heterozygosity (Ho) ranges from 0.1656 to 0.7808 with an average of 0.3035; the Nei’s gene diversity index (H) ranges from 0.2170 to 0.8224 with an average of 0.6863; Shannon-Wiener The index (I) ranges from 0.5186 to 1.8597 with an average of 1.4517. (4) UPGMA clustering of 51 materials showed that the test materials could be divided into three groups when the genetic distance was 0.856. The experiment shows that the genetic diversity of the ‘Zuijinxiang’ radiation variation germplasm resources is rich. In addition, SCoT molecular marker technology can distinguish the materials with close genetic distance, and can be used for early identification techniques of grape mutant materials. This study provides a theoretical basis for the development of excellent mutant germplasm of ‘Zuijinxiang’ grapes.

Key words:
grape; SCoT markers; genetic diversity; cluster analysis

[1] E-mail: 1150422578@qq.com. ^*Corresponding author.

No.	Name	No.	Name	No.	Name	No.	Name
1	C1-1	14	C2-3	27	C4-4	40	C6-4
2	C1-3	15	C2-5	28	C4-5	41	C6-5
3	C1-5	16	C2-7	29	C4-6	42	C6-6
4	C1-6	17	C2-9	30	C4-9	43	C6-7
5	C1-9	18	C2-12	31	C4-10	44	C6-8
6	C1-10	19	C2-13	32	C5-2	45	C6-9
7	C1-11	20	C2-15	33	C5-3	46	C6-11
8	C1-12	21	C2-17	34	C5-6	47	E2
9	C1-13	22	C3-1	35	C5-7	48	1
10	C1-14	23	C3-4	36	C5-9	49	2
11	C1-15	24	C3-11	37	C5-10	50	4
12	C1-16	25	C3-12	38	C6-1	51	Zuijinxiang
13	C2-1	26	C4-3	39	C6-3

Primer	Sequence	Total band number	Polymorphic band number	Percentage of polymorphic bands(%)
SCoT1	CAACAATGGCTACCACCA	5	5	100
SCoT2	CAACAATGGCTACCACCC	6	4	66.7
SCoT3	CAACAATGGCTACCACCG	5	4	80
SCoT4	CAACAATGGCTACCACCT	6	5	83.3
SCoT5	CAACAATGGCTACCACGA	4	3	75
SCoT6	CAACAATGGCTACCACGC	8	8	100
SCoT7	CAACAATGGCTACCACGG	5	5	100
SCoT8	CAACAATGGCTACCACGT	6	4	66.7
SCoT9	CAACAATGGCTACCAGCA	5	3	60
SCoT10	CAACAATGGCTACCAGCC	6	6	100
SCoT11	AAGCAATGGCTACCACCA	8	5	62.5
SCoT12	ACGACATGGCGACCAACG	7	5	71.4
SCoT13	ACGACATGGCGACCATCG	6	6	100
SCoT14	ACGACATGGCGACCACGC	5	3	60
SCoT15	ACGACATGGCGACCGCGA	6	4	66.7
SCoT16	ACCATGGCTACCACCGAC	7	5	71.4
SCoT17	ACCATGGCTACCACCGAG	6	6	100
SCoT18	ACCATGGCTACCACCGCC	4	4	100
SCoT19	ACCATGGCTACCACCGGC	8	7	87.5
SCoT20	ACCATGGCTACCACCGCG	6	5	83.3
SCoT21	ACGACATGGCGACCCACA	4	4	100
SCoT22	AACCATGGCTACCACCAC	8	6	75
SCoT23	CACCATGGCTACCACCAG	6	4	66.7
SCoT24	CACCATGGCTACCACCAT	7	6	85.7
SCoT25	ACCATGGCTACCACCGGG	7	5	71.4
SCoT26	ACCATGGCTACCACCGTC	4	4	100
SCoT27	ACCATGGCTACCACCGTG	6	5	83.3
SCoT28	CCATGGCTACCACCGCCA	8	5	62.5
SCoT29	CCATGGCTACCACCGGCC	6	5	83.3
SCoT30	CCATGGCTACCACCGGCG	8	6	75
SCoT31	CCATGGCTACCACCGCCT	7	5	71.4
SCoT32	CCATGGCTACCACCGCAC	7	4	57.1
SCoT33	CCATGGCTACCACCGCAG	4	4	100
SCoT34	ACCATGGCTACCACCGCA	6	5	83.3
SCoT35	CATGGCTACCACCGGCCC	8	6	75
SCoT36	GCAACAATGGCTACCACC	6	4	66.7
Total		221	175	-
Mean		6.1	4.9	80.3

Primer	Na	Ne	He	Ho	H	I
SCoT1	5	2.5525	0.6142	0.3858	0.6082	1.2308
SCoT2	6	3.9186	0.7547	0.2453	0.7448	1.5299
SCoT3	5	3.4792	0.7235	0.2765	0.7126	1.3790
SCoT4	6	3.1038	0.6845	0.3155	0.6778	1.4357
SCoT5	4	3.9512	0.7683	0.2317	0.7469	1.3801
SCoT6	8	3.4473	0.7169	0.2831	0.7099	1.6352
SCoT7	5	1.2781	0.2198	0.7802	0.2176	0.5186
SCoT8	6	1.2772	0.2192	0.7808	0.2170	0.5220
SCoT9	5	3.1494	0.6916	0.3084	0.6825	1.3002
SCoT10	6	2.3656	0.5830	0.4170	0.5773	1.2511
SCoT11	8	3.2717	0.7012	0.2988	0.6943	1.5551
SCoT12	7	4.4454	0.7923	0.2077	0.7750	1.6909
SCoT13	6	4.9388	0.8161	0.1839	0.7975	1.6687
SCoT14	5	4.6667	0.7971	0.2029	0.7857	1.5716
SCoT15	6	3.0475	0.6785	0.3215	0.6719	1.4300
SCoT16	7	5.1914	0.8215	0.1785	0.8074	1.8081
SCoT17	6	3.0403	0.6777	0.3223	0.6711	1.4032
SCoT18	4	3.9670	0.7681	0.2319	0.7479	1.3820
SCoT19	8	3.0781	0.6818	0.3182	0.6751	1.5571
SCoT20	6	2.4006	0.5892	0.4108	0.5834	1.2443
SCoT21	4	3.3543	0.7108	0.2892	0.7019	1.2767
SCoT22	8	5.3473	0.8259	0.1741	0.8130	1.8597
SCoT23	6	2.9624	0.6690	0.3310	0.6624	1.4083
SCoT24	7	5.6322	0.8344	0.1656	0.8224	1.8255
SCoT25	7	5.1914	0.8215	0.1785	0.8074	1.8081
SCoT26	4	3.9512	0.7683	0.2317	0.7469	1.3801
SCoT27	6	3.9186	0.7547	0.2453	0.7448	1.5299
SCoT28	8	5.3473	0.8259	0.1741	0.8130	1.8597
SCoT29	6	3.1038	0.6845	0.3155	0.6778	1.4357
SCoT30	8	3.2717	0.7012	0.2988	0.6943	1.5551
SCoT31	7	4.4454	0.7923	0.2077	0.7750	1.6909
SCoT32	7	5.1914	0.8215	0.1785	0.8074	1.8081
SCoT33	4	3.3543	0.7108	0.2892	0.7019	1.2767
SCoT34	6	2.3656	0.5830	0.4170	0.5773	1.2511
SCoT35	8	3.0781	0.6818	0.3182	0.6751	1.5571
SCoT36	6	2.4006	0.5892	0.4108	0.5834	1.2443
Mean	6.1389	3.5968	0.6965	0.3035	0.6863	1.4517

Brasil

Brasil

Analysis on genetic diversity of 51 Grape germplasm resources

Análise da diversidade genética da videira 51

ABSTRACT: