Degree of moisture in seeds for the cryopreservation of orchids native to Brazil

Ferrari, Edilene Aparecida Preti; Colombo, Ronan Carlos; Faria, Ricardo Tadeu de; Neves, Carmen Silvia Vieira Janeiro; Vero, Franciele da Silva

doi:10.1590/0103-8478cr20180498

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

BIOLOGY • Cienc. Rural 50 (8) • 2020 • https://doi.org/10.1590/0103-8478cr20180498 linkcopy

Degree of moisture in seeds for the cryopreservation of orchids native to Brazil

Grau de umidade para criopreservação de sementes de orquídeas nativas do Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.

Key words:
liquid nitrogen; freezing; survival; Cattleya labiata Lindl.; Miltonia regnellii Rchb.f.

vertical_align_top show_chart

more_horiz close
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

[TFN1] ^* Averages followed by the same lower-case letters in the column do not significantly differ by the Tukey test at 5%.

[TFN2] ^** Initial viability of C. labiata seeds = 98%.

[TFN3] ^*** Initial viability of M. regnellii seeds = 47%.

Moisture degree (%)	------------------Cattleya labiata Lindl.-----------------		-----------------Miltonia regnellii Rchb.f.---------------
	VS (%)^**	PS (%)	VS (%)^***	PS (%)
4	95 a^*	97 a^*	68 a^*	88 a*
6	88 b	100 a	53 b	100 a
8	69 c	96 a	19 c	82 a
10	62 d	100 a	13 c	93 a
12	-	-	-	-
15	-	-	-	-
CV%	5.49	5.57	30.05	25.07

Moisture degree (%)	------------------Cattleya labiata Lindl.------------------			-----------------Miltonia regnellii Rchb.f.------------------
	FM (g)	DM (g)	NS	FM (g)	DM (g)
4	0.073 a	0.006 ab	1.37 a	0.024 a	0.0027 ab
6	0.077 a	0.007 a	1.22 a	0.026 a	0.0034 a
8	0.052 a	0.005 ab	1.38 a	0.014 a	0.0022 b
10	0.052 a	0.004 b	1.29 a	0.022 a	0.0027 ab
12	-	-	-	-	-
15	-	-	-	-	-
CV%	34.62	30.06	38.13	46.03	31.46