The efficiency of three monolayers (continuous lineage - Madin and Darby Bovine Kidney/ MDBK; primary lineage - bovine celisfrom uterus and oviduct) hás been tested to verify the celular specificity through development of two cells embryos until compact morulaes stage in a coculture system without continuous CO2 flow. The selected mouse embryos (n=343) were randomiy divided into three experimental groups andplaced in closed culture tubes mantained aí 37°C during 72 hours. After the co-culture period, the porcentages of compact morulaes were 87.7% in oviduct cells, 86.2% in uterus monolayer and 88.3% in MDBK cells. It was not observed significative difference between these results and it is possible to condude that celular specificity is not importam to enhance the In vitro development of Mus musculus embryos.
embryo; Mus musculus; oviduct cells; uterine cells; MDBK cells
