Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

Germinação de sementes e atividade de enzimas antioxidantes em plântulas de grama forquilha diplóide e tetraploide sob restrição hídrica

ABSTRACT:

The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG₆₀₀₀), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG₆₀₀₀ reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).

Key words:
Paspalum notatum Flügge; ecotype; polyethylene glycol; drought stress; oxidative stress