The production of aflatoxin in rice by three isolates of Aspergillus flavus was investigated for different culture conditions (temperature and incubation time) and previous inoculum (YES- Yeast Extrat Sucrose and CYA- Czapeck Yeast Extrat). All strains withdrawn from the previous inoculum medium YES and inoculated in the rice in temperature of 25°C/18 days and 20°C/14 days, produced aflatoxin B1. The medium CYA had lower performance since the three strains tested did not produce aflatoxin B1 in the combination 20°C/14 days. At 25°C/11 days of incubation time aflatoxin was not detectable.
Aspergillus flavus; CYA; YES; incubation time; temperature; rice
