Primer designation |
Sequencing 5' 3' |
Species |
Targets |
Amplicon size (bp) |
Reference for thermal profile |
GPO3 |
GGG AGC AAA CAG GAT TAG ATA CCC T |
Mollicutes |
16S rRNA |
270 |
VAN KUPPEVELD et al. (1992VAN KUPPEVELD, F.J.M. et al. Genus- and species-specific identification of Mycoplasmas by 16S rRNA amplification. Applied and Environmental Microbiology, v.58, n.8, p.2606-2615, 1992. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC195828/pdf/aem00049-0274.pdf >. Accessed: Jul. 05, 2020. PMID: 1381174. https://www.ncbi.nlm.nih.gov/pmc/article...
) |
MGSO |
TGC ACC ATC TGT CAC TCT GTT AAC CTC |
Mbo F |
CCT TTT AGA TTG GGA TAG CGG ATG |
M. bovis
|
16S rRNA |
360 |
CHÁVEZ-GONZALEZ et al. (1995CHÁVEZ-GONZÁLEZ, Y. R. et al. In vitro amplification of the 16s rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Veterinary Microbiology, v.47, n. 1-2, p.183-190, 1995. Available from: <Available from: https://www.sciencedirect.com/science/article/pii/037811359500058I >. Accessed: Jun. 05, 2020. doi: 10.1016/0378-1135(95)00058-I. https://www.sciencedirect.com/science/ar...
) |
Mbo R |
CCG TCA AGG TAG CAT CAT TTC CTA T |
Myc 1F |
CAC CGC CCG TCA CAC CA |
M. arginini
|
16S/23S rRNA |
312 |
CHALKER et al. (2004CHALKER, V. J. et al. Mycoplasmas associated with canine infectious respiratory disease. Microbiology, v.150, n.10, p.3491-3497, 2004. Available from: <Available from: https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26848-0#tab2 >. Acessed: Jun 05, 2020. doi: 10.1099/mic.0.26848-0. https://www.microbiologyresearch.org/con...
) |
M. arginini
|
GTT GTA TGA CCT ATT GTT GTC |
Mak F |
CCG TCA AGG TAG CAT CAT TTC CTA T |
M. alkalescens
|
16S rRNA |
704 |
KOBAYASHI et al. (1998KOBAYASHI, H. et al. In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. Journal of Veterinary Medical Science, v.6, n.12, p.1299-1303, 1998. Available from: <Available from: https://www.jstage.jst.go.jp/article/jvms/60/12/60_12_1299/_pdf/-char/en >. Accessed: Jun. 05, 2020. doi: 10.1292/jvms.60.1299. https://www.jstage.jst.go.jp/article/jvm...
) |
Mak R |
AGA GTC CTC GAC ATG ACT CG |
Mbg F |
CGT AGA TGC CGC ATG GCA TTT ACG G |
M. bovigenitalium
|
16S rRNA |
312 |
KOBAYASHI et al. (1998KOBAYASHI, H. et al. In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. Journal of Veterinary Medical Science, v.6, n.12, p.1299-1303, 1998. Available from: <Available from: https://www.jstage.jst.go.jp/article/jvms/60/12/60_12_1299/_pdf/-char/en >. Accessed: Jun. 05, 2020. doi: 10.1292/jvms.60.1299. https://www.jstage.jst.go.jp/article/jvm...
) |
Mbg R |
CAT TCA ATA TAG TGG CAT TTC CTA C |
Mbr F |
GCT GAT AGA GAG GTC TAT CG |
M. bovirhinis
|
16S rRNA |
316 |
KOBAYASHI et al. (1998KOBAYASHI, H. et al. In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. Journal of Veterinary Medical Science, v.6, n.12, p.1299-1303, 1998. Available from: <Available from: https://www.jstage.jst.go.jp/article/jvms/60/12/60_12_1299/_pdf/-char/en >. Accessed: Jun. 05, 2020. doi: 10.1292/jvms.60.1299. https://www.jstage.jst.go.jp/article/jvm...
) |
Mbr R |
ATT ACT CGG GCA GTC TCC |
ACH3 |
AGC CGG ACT GAG AGG TCT AC |
A. laidlawii
|
16S rRNA |
505 |
DUSSURGET & ROULLAND-DUSSOIX (1994DUSSURGET, O.; ROULLAND-DUSSOIX, D. Rapid, sensitive PCR- based detection of Mycoplasmas in stimulated samples of animal sera. Applied and Environmental Microbiology, v.60, n.3, p.953-959, 1994. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC201416/pdf/aem00020-199.pdf >. Accessed: Jun. 05, 2020. PMID: 8161186. https://www.ncbi.nlm.nih.gov/pmc/article...
) DUSSURGET, O.; ROULLAND-DUSSOIX, D. Rapid, sensitive PCR- based detection of Mycoplasmas in stimulated samples of animal sera. Applied and Environmental Microbiology, v.60, n.3, p.953-959, 1994. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC201416/pdf/aem00020-199.pdf >. Accessed: Jun. 05, 2020. PMID: 8161186. https://www.ncbi.nlm.nih.gov/pmc/article...
|
UNI |
TAA TCC TGT TTG CTC CCC AC |