Effects and mechanisms of water-soluble <i>Semen</i> cassiae polysaccharide on retinitis pigmentosa in rats

HE, Suiqin; MA, Xiang; MENG, Qinggui; LU, Jianmin; QIN, Xiuhong; FANG, Shifeng; MA, Cong

doi:10.1590/fst.32718

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Original Article • Food Sci. Technol 40 (1) • Jan-Mar 2020 • https://doi.org/10.1590/fst.32718 copy

Effects and mechanisms of water-soluble Semen cassiae polysaccharide on retinitis pigmentosa in rats

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study aimed to investigate the effects and mechanisms of water-soluble Semen cassiae polysaccharide (SCP) on retinitis pigmentosa (RP) in rats. One hundred rats were randomly divided into control, model, and 50, 100 and 200 mg/kg SCP groups. The 50, 100 and 200 mg/kg SCP groups were treated with 50, 100 and 200 mg/kg SCP for 5 days, respectively. On the sixth day, the model, and 50, 100 and 200 mg/kg SCP groups were treated with N-nitroso-N-methylurea (MNU). After 12 h and 5 days, the electroretinogram, retinal thickness, apoptosis of retinal photoreceptor cells, and expressions of apoptosis related protein in retina tissue were determined. Results showed that, after 12 h and on 5 days from MNU treatment, compared with model group, the a-wave and b-wave amplitude and retinal thickness in SCP groups were significantly increased, the apoptosis rate of retinal photoreceptor cells was significantly decreased, the Bcl-2 protein expression level in retinal tissue was significantly increased, and the Bax and Caspase-3 protein level were significantly decreased (all P < 0.05). In conclusion, SCP has obvious protective effects on RP in rats. The mechanism may be related to it regulation of Bcl-2, Bax and Caspase-3 protein expression in retina tissue.

Keywords:
polysaccharide; Semen cassiae; retinitis pigmentosa

Group	24 h		5 day
Group	a-Wave (μV)	b-Wave (μV)	a-Wave (μV)	b-Wave (μV)
Control	99.7 ± 9.0	198.8 ± 20.5	101.5 ± 12.9	195.7 ± 21.9
Model	27.6 ± 5.7^a a P < 0.05 compared with control group;	69.2 ± 11.0^a	16.1 ± 4.0^a^* * P < 0.05 compared with 24 h. SCP = Semen cassiae polysaccharide; ERG = electroretinogram.	33.3 ± 8.9^a*
50 mg/kg SCP	33.5 ± 7.4^a	73.6 ± 9.6^a	17.3 ± 4.9^a*	55.4 ± 9.0^ab*
100 mg/kg SCP	56.7 ± 6.1^a^b b P < 0.05 compared with model group; ^c	140.2 ± 26.0^abc	34.7 ± 9.9^abc*	84.2 ± 13.4^abc*
200 mg/kg	69.3 ± 8.0^ab^c c P < 0.05 compared with 50 mg/kg SCP group; ^d	162.8 ± 24.2^abc	55.0 ± 12.0^abc^d d P < 0.05 compared with 100 mg/kg SCP group; *	121.0 ± 15.7^abcd*

Group	24 h		5 day
Group	Full thickness (μm)	Outer layer thickness (μm)	Full thickness (μm)	Outer layer thickness (μm)
Control	122.1 ± 4.3	56.2 ± 2.8	123.6 ± 3.2	55.1 ± 2.0
Model	111.3 ± 3.0^a a P < 0.05 compared with control group;	21.6 ± 1.0^a	68.3 ± 2.5^a^* * P < 0.05 compared with 24 h. SCP = Semen cassiae polysaccharide.	2.4 ± 0.9^a*
50 mg/kg SCP	114.2 ± 1.8^a^b b P < 0.05 compared with model group;	24.6 ± 2.0^ab	77.2 ± 1.8^ab*	23.4 ± 1.4^ab
100 mg/kg SCP	116.4 ± 1.6^ab^c c P < 0.05 compared with 50 mg/kg SCP group;	49.2 ± 2.3^abc	94.4 ± 2.1^abc*	47.7 ± 2.3^abc
200 mg/kg	121.8 ± 2.0^bc^d d P < 0.05 compared with 100 mg/kg SCP group;	53.4 ± 2.6^abcd	120.8 ± 3.0^bcd	49.8 ± 3.0^abc*

Group	24 h	5 day
Control	0.5 ± 0.1	0.3 ± 0.0
Model	24.3 ± 3.2^a a P < 0.05 compared with control group;	12.3 ± 2.0^a^* * P < 0.05 compared with 24 h. SCP = Semen cassiae polysaccharide.
50 mg/kg SCP	18.3 ± 2.2^a^b b P < 0.05 compared with model group;	11.8 ± 1.9^a*
100 mg/kg SCP	5.1 ± 1.9^ab^c c P < 0.05 compared with 50 mg/kg SCP group;	3.2 ± 1.0^abc*
200 mg/kg	4.5 ± 1.2^abc	2.7 ± 0.5^abc*

Group	24 h	5 day
Control	1.3 ± 0.2	1.3 ± 0.1
Model	1.1 ± 0.2^a a P < 0.05 compared with control group;	1.1 ± 0.1^a
50 mg/kg SCP	1.2 ± 0.2^a	1.1 ± 0.1^a
100 mg/kg SCP	1.2 ± 0.2^a	1.3 ± 0.1^bc
200 mg/kg	1.3 ± 0.1^a^b b P < 0.05 compared with model group;	1.3 ± 0.2^b^c c P < 0.05 compared with 50 mg/kg SCP group. SCP = Semen cassiae polysaccharide; Bcl-2 = B-cell lymphoma-2.

Group	24 h	5 day
Control	0.3 ± 0.1	0.3 ± 0.0
Model	1.1 ± 0.1^a a P < 0.05 compared with control group;	1.0 ± 0.1^a
50 mg/kg SCP	1.0 ± 0.1^a	0.9 ± 0.1^ab*
100 mg/kg SCP	0.8 ± 0.2^a^b b P < 0.05 compared with model group; ^c	0.6 ± 0.1^abc*
200 mg/kg	0.4 ± 0.1^b^c c P < 0.05 compared with 50 mg/kg SCP group; ^d	0.4 ± 0.1^abc^d d P < 0.05 compared with 100 mg/kg SCP group. SCP = Semen cassiae polysaccharide; Bax = Bcl-2 associated X.

Group	24 h	5 day
Control	0.2 ± 0.0	0.3 ± 0.0
Model	0.4 ± 0.1^a	0.4 ± 0.1^a
50 mg/kg SCP	0.3 ± 0.1^a	0.3 ± 0.0^a
100 mg/kg SCP	0.3 ± 0.0^a a P < 0.05 compared with control group; ^bc	0.2 ± 0.0^b^c c P < 0.05 compared with 50 mg/kg SCP group;
200 mg/kg	0.2 ± 0.0^b b P < 0.05 compared with model group; ^c^d d P < 0.05 compared with 100 mg/kg SCP group. SCP = Semen cassiae polysaccharide; Caspase-3 = Cysteinyl aspartate-specific proteinase-3.	0.3 ± 0.1^bc

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[1] *Corresponding author: maxiangdl@126.com