Procedures for intralaboratory validation of an ELISA kit were adopted to verify its efficiency in aflatoxin M1 detection and quantification in milk. Assays were accomplished with standard solutions provided with the kit, spiked samples and naturally contaminated samples. The mean recovery values obtained for the kit standard solutions were between 85.6 and 114.8%, with coefficient of variation ranging from 11.6 to 23.1%. Working with spiked samples, a concentration range for quantitative analysis (0.019 to 0.090µg/L) was defined. The occurrence of aflatoxin M1 in 110 milk samples from the State of Minas Gerais was investigated. Five of the 27 positive samples in the screening for ELISA were confirmed by thin layer chromatography.
milk; aflatoxin M1; validation; ELISA
