A method for aflatoxin M1 determination in milk by thin layer chromatography was otimized and in house validated. Steps of extraction, clean-up, detection and quantification by visual and densitometric analysis were standardized. In method validation were employed standard solutions and spiked samples in levels varying from 0.027µg/L to 0.970µg/L. Linearity, specificity, trueness, precision, detection and quantification limits were the parameters evaluated. Average recovery values in quantification range were from 84.2% to 99.0% for visual analysis and from 85.2% to 105.2% for densitometric analysis, with CVs values from 2.3% to 9.8% and from 3.6% to 13.9%, respectively. The detection limit was 0.036µg/L and the quantification 0.045µg/L, according to national and international regulations for aflatoxin M1 in milk.
milk; aflatoxin M1; validation; TLC
