Lactobacillus casei enhances the apoptosis inducing effect of geniposide on U87 human glioma cells in vitro

Abstract

The goal of this work was to clarify the mechanism of action of Lactobacillus casei on genipin in triggering glioma apoptosis. DPPH and ABTS free radicals could be successfully eliminated by geniposide, LC-NBRC101979 (Lactobacillus casei), and geniposide + LC-NBRC101979. Geniposide and LC-NBRC101979 had effects that were stronger when combined than when taken alone. Geniposide, LC-NBRC101979, and Geniposide + LC-NBRC101979 did not have any detrimental effects on healthy epithelial cells HBE, but they greatly decreased the growth of U87 glioma cells, according to cell studies. Additionally, the combination of geniposide and LC-NBRC101979 had a stronger inhibitory impact than either drug alone. Results from qPCR revealed that in U87 cells, geniposide, LC-NBRC101979, and eniposide + LC-NBRC101979 could up-regulate caspase-3, COX-2, p53, c-myc, p21 mRNA expression and down-regulate Bcl-2, NF-κB expression. According to the results of the study on the effects of oxidative stress on cells, geniposide, LC-NBRC101979, and geniposide combined with LC-NBRC101979 can decrease the levels of MDA, LDH, and NO in normally damaged epithelial cells caused by oxidative stress and increase the levels of CAT, SOD, GSH-Px, and T-AOC. According to HPLC data, LC-NBRC101979 was able to convert the majority of geniposide into geniposide. It is clear that LC-NBRC101979 may enhance geniposide's inhibitory impact on glioma.

Keywords:
Gardenia jasminoides; gardenia; genipin; Lactobacillus casei; glioma