Abstract
Cervical cancer ranks third in cancer incidence worldwide and is the most frequent gynecological cancer in developing countries. This study aimed to investigate the effect of crocin on the resistance of HELA/Ara-C cells to the chemo-agent of Ara-C. HELA/Ara-C cells which was defined as Ara-C-resistant HELA cell line, were treated with crocin. After that, cell proliferation, apoptosis, and cell cycle assay were examined by MTT assay, AO/EB staining, and flow cytometry, respectively. In this study, we found that crocin combining with Ara-C significantly inhibited the proliferation HELA/Ara-C cell, and this inhibitory effect was time dependent. Moreover, crocin combining with Ara-C could induced apoptosis and cell cycle arrest of HELA/Ara-C Cells. Further then, HELA/Ara-C cells were transfected with the mature miRNA-181s mimic and the control miRNA mimic, respectively. Quantitative RT-PCR results demonstrated that compared with HELA/Ara-C Cells +Ara-C group, miRNA-181a was upregulated 1.5-fold in HELA/Ara-C Cells + crocin group and 1.7-fold in HELA/Ara-C Cells and crocin +Ara-C group, respectively. The other miRNA-181s showed no significant differences. In summary, this was the first study to show that miRNA-181a might be involved in the development of crocin induced reversing Ara-C resistance in HELA/Ara-C cell lines.
Keywords:
MiRNAs; crocin; HELA/Ara-C cells
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