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Production of extracellular enzymes by Crinipellis perniciosa

Strains of Crinipellis perniciosa isolated from cocoa (Theobromae cacao), cupuassu (T. grandiflorum) and wild solanaceous were tested for their ability to produce extracellular enzymes which degrade cellulose, starch, lipids and lignin. The production of all enzymes was examined in solid media and was estimated based on the intensity of color or on the diameter of halos formed on the media. Variability was detected among the isolates in their capacity to produce cellulolytic enzymes, amylase, lypase, phenol-oxidase, peroxidase and esterase. All isolates presented high level of activity, regarding proteolytic enzymes. On the other hand, none of the isolates produced pectinase, urease and acid phosphatase. The roles of lytic enzymes of C. perniciosa on pathogenesis and on basidiome production are discussed.

Theobromae cacao; T. grandiflorum; variability; solanaceus


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