Nodal segment explants of potato (Solanum tuberosum L.) cv Achat were excised from in vitro growing plants, and transformed with Agrobacterium tumefaciens LBA4404 carrying the binary vector pBI121 with npt II and gus-intron genes. The regeneration of putative transformants was done in medium supplemented with 50 mg.L-1 of kanamycin. Putative transformants expressing kanamycin resistance and b-glucuronidase activity were identified. PCR analysis of these plants showed amplification of the npt II in gus+ plants. Southern blotting hybridization revealed the insertion of at least one copy of the npt II gene.
Solanum tuberosum; regeneration; transgenic plants
