Agrobio is a biofertilizer prepared from fresh cattle manure, water, molasses and minerals, fermented under room temperature in open containers during 56 days. This product has been widely used by organic and conventional farmers throughout Rio de Janeiro State, Brazil, to control several plant pathogens of various crops. The antibiotic effect of different concentrations of Agrobio against Xanthomonas euvesicatoria, in vitro vas evaluated. Four bioassays were performed with three isolates of the bacterium. Agrobio was tested in three preparation stages (35, 85 and 115 days of fermentation) and applied raw, filtered (0,22 µm) or autoclaved (120ºC/20 minutes). The effect of the treatments was assessed by the diameter of the inhibition growth halo on Petri dishes. Agrobio presented bacteriostatic effect on X. euvesicatoria regardless of the sample age or pre-treatment. Growth inhibition occurred above 5% concentration, indicating the feasibility of Agrobio, as a bacterial leaf spot control agent, in early fermentation stages (35 days). Field experiments are necessary to confirm these results.
Capsicum annuum L.; Xanthomonas euvesicatoria; antibiosis
