The Ananas comosus var. erectifoliusis an ornamental pineapple cultivar which greatly interests Brazilians and foreign landscapers and flower producers for being an exotic and rustic tropical ornamental plant. The market demand for high quality of cuttings requires efficient methods of propagation and in this context the tissue culture stands out as a viable alternative to obtain plants with genetic and phytossanitary quality in a short time. In the present work we studied the influence of concentrations of BAP (0; 0.5; 1.0; 1.5 mg L-1) and NAA (0; 0.12; 0.24; 0.48 mg L-1) in the MS medium culture within 0; 2.5; 5.0; 7.5 g L-1 of agar, in order to establish an invitro protocol for multiplication and rooting of ornamental pineapple. Plantlets with 1.5±0.5 cm already established in vitro, extracted from buds of ornamental pineapple fruits crown were inoculated aseptically in flasks. After inoculation the plantlets were kept in a growth room at 26±1ºC, 35 ìmol m-2 s-1 irradiance and a 16-hour photoperiod. After 45 days we observed that the multiplication of ornamental pineapple is viable at liquid MS medium with BAP 1.5 mg L-1 and the rooting is also increased in liquid MS medium in absence of growth regulators.
Ananas comosus var. erectifolius; Bromeliacea; tissue culture; rooting; multiplication
