In this work we characterized the genetic-molecular diversity by RAPD markers of the main ten strawberry cultivars cultivated in Brazil: Aromas, Bürkley, Camarosa, Campinas, Dover, Milsei-Tudla, Oso Grande, Santa Clara, Sweet Charlie and Vila Nova. The DNA was extracted from mature leaves, the RAPD analysis were carried out with 26 primers and the amplification products were separated by electrophoreses. The coefficient of Dice was used to estimate the genetic similarity among the cultivars and the UPGMA method to obtain the phenogram using NTSYS software. Bands were amplified with 19 primers, and showed polymorphism with 14. Out of 116 bands, 84 were polymorphic. The cultivars were classified in two main groups: industry cultivars (Bürkley, Santa Clara and Vila Nova) and fresh fruit market cultivars (other cultivars). The genetic similarity was lesser in the group of cultivars for fresh market (44-74%), in which the different clones were used as parents. The cultivars Santa Clara and Vila Nova, used for processing, showed the higher genetic similarity (98%), both cultivars were obtained at breeding program of Embrapa Clima Temperado, and are originated from the same parental clones ('Konvoy-Cascata' and 'Lassen'). Standard RAPD markers were established to genetic characterization of ten strawberry cultivars.
Fragaria x ananassa Duch.; RAPD; fingerprint; molecular marker
