The respiratory rate and ethylene production were evaluated in whole and minimally processed kale leaves and, the influence of harvest time, of fast cooling and sanitization temperature on the conservation of the minimally processed product. Totally developed kale leaves were harvested at 7 and 14 o'clock in the field, during the years 1996 and 2000. Processing was carried out soon after harvest and subsequent refrigeration (6±1ºC for 8 h). Samples (200 g) were conditioned in multilayer poliolefina packing, stored at 5±2ºC for 15 days and evaluate for vitamin C and polifenoloxidase (PPO) activity. The respiratory rate and production of ethylene were determined in whole leaves as well as in minimally processed ones, packed in plastic trays and glass jars hermetically closed. The packs were stored during 24 hours under temperatures of 1; 5; 10 and 25ºC. The respiratory rate and the ethylene production increased immediately after the detachment of the leaves from the mother plant. Cutting increased the respiratory rate in approximately two-folds. Increasing of the temperature affected significantly (P<0,05%) the respiratory rate and the production of ethylene. Fast cooling reduced the metabolism, but did not result in greater storage time. The low temperature during sanitization is important for the conservation of the minimally processed kale. The harvest time influenced significantly (P<0,05%) the respiratory rate and PPO activity of the minimally processed kale, but did not influence the storage time.
Brassica oleracea L. var. acephala; respiratory rate; ethylene; poliphenol oxidase; ascorbic acid; storage
