This work was carried out at the Tissue Culture Laboratory at Embrapa Clima Temperado Pelotas-RS, Brazil, to evaluate the influence of acetyl salycilic acid (ASA), sucrose and temperature in the in vitro storage of potato shoot cvs. Baronesa and Santo Amor. The shoot segments were inoculated on a medium containing MS salts, vitamins, myo-inositol (100 mg.L-1), agar (6.0 g.L-1) and sucrose at 10, 30 and 50 g.L-1 combined with acetyl salicylic acid at 0, 3, 6, 9 and 12 mg.L-1. After inoculation the material was kept at 10 or 25°C. The experiment was designed in a factorial A x B x C x D [temperature (2) x sucrose (3) x ASA (5) x cultivars (2)] and displayed in a randomized block with four replicates being each explant one plot. The shoot growth was studied and the data were compared by the Duncan test and by polinomial regression. Evaluation took place every 15 days and the last one was done 180 days after inoculation. The best results were obtained using in vitro storage with the shoots kept at 10°C, on a medium containing to 30 g.L-1 sucrose.
Solanum tuberosum L.; tissue culture
