<i>Rhizophagus clarus</i> controls <i>Meloidogyne javanica</i> and enhances the activity of defense-related enzymes in tomato

Silva, Monique TR e; Silva, Beatriz de A e; Alberton, Odair; Schwengber, Raiane P; Dias-Arieira, Claudia R

doi:10.1590/s0102-0536-20220205

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Research • Hortic. Bras. 40 (2) • Apr-Jun 2022 • https://doi.org/10.1590/s0102-0536-20220205 copy

Rhizophagus clarus controls Meloidogyne javanica and enhances the activity of defense-related enzymes in tomato

Rhizophagus clarus controla Meloidogyne javanica e aumenta a atividade de enzimas relacionadas a defesa em tomateiro

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Root-knot nematodes (Meloidogyne spp.) cause severe damage to tomato crop. This study aimed to investigate the biocontrol potential of the arbuscular mycorrhizal fungus Rhizophagus clarus compared with that of the fungi Trichoderma harzianum and Pochonia chlamydosporia against Meloidogyne javanica in tomato and assess the activity of defense-related enzymes in mycorrhizal roots. Three experiments were carried out to evaluate mycorrhizal colonization, nematode penetration, development, and reproduction, plant growth, and enzyme activity in mycorrhizal roots. We observed that R. clarus colonization of tomato roots increased with time. Although R. clarus did not prevent nematode penetration or development in roots, it was efficient in reducing total nematode numbers. R. clarus, T. harzianum and P. chlamydosporia treatments reduced nematode reproduction by 56.5, 54.4, and 56.9%, respectively, compared with the control. Moreover, R. clarus increased tomato shoot weight and phenylalanine ammonia-lyase and peroxidase activities.

Keywords:
Trichoderma harzianum; Pochonia chlamydosporia; root-knot nematodes; mycorrhiza; resistance induction

DAI	Treatment	J4	Females	Total
5	Control	0.0 b	0.0 b	1.25 c
	Rhizophagus clarus	0.0 b	0.0 b	1.0 c
	Trichoderma harzianum	0.0 b	0.0 b	0.25 c
	Pochonia chlamydosporia	0.0 b	0.0 b	0.0 c
10	Control	0.0 b	0.0 b	2.25 c
	R. clarus	0.0 b	0.0 b	7.50 c
	T. harzianum	0.0 b	0.0 b	0.50 c
	P. chlamydosporia	0.0 b	0.0 b	1.0 c
15	Control	15.2 a	3.2 a	38.0 ab
	R. clarus	11.5 a	2.2 a	38.5 a
	T. harzianum	1.5 b	0.0 b	19.0 bc
	P. chlamydosporia	3.2 b	0.0 b	14.7 c
MSD/SE		3.93/0.79	1.51/0.30	19.13/3.89
Significance	DAI	F = 130.131**	F = 26.889**	F = 59.857**
	Treatment	F = 22.656 **	F = 9.556**	F = 5.840**
	DAI × Treatment	F = 22.656**	F = 9.556**	F = 2.808*

Treatment	Total number	Population density	RF	Height	SFW	SDW	RFW
Control	30938 a	4250^ns	15.47 a	46.96^ns	12.81 b	2.68 b	7.44^ns
Rhizophagus clarus	13470 b	2248 ^ns	6.73 b	48.91 ^ns	18.78 a	3.97 a	6.01 ^ns
Trichoderma harzianum	14115 b	2899 ^ns	7.06 b	44.41 ^ns	12.96 b	2.65 b	5.44 ^ns
Pochonia chlamydosporia	13345 b	2736 ^ns	6.67 b	46.70 ^ns	11.49 b	2.28 b	6.07 ^ns
MSD/SE	26.9/6.8	19.7/4.9	0.6/0.1	0.4/0.1	0.4/0.1	0.2/0.07	0.4/0.1

Associação Brasileira de Horticultura Embrapa Hortaliças, C. Postal 218, 70275-970 Brasília-DF, Tel. (61) 3385 9099, Tel. (81) 3320 6064, www.abhorticultura.com.br - Vitoria da Conquista - BA - Brazil
E-mail: associacaohorticultura@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[TFN1] Means in a column followed by the same letter do not differ at p<0.05 by Tukey’s test. MSD= minimum significant difference; SE= standard error; *significant at p<0.05; **significant at p<0.01.