Knowledge of arbuscular mycorrhizal fungi (AMF) communities depends of spores, which may not be available at the moment of collection. The main objective of this paper is the determination of AMF species diversity from direct collection carried out in the field and multiplication in pots. Five soil samples from three areas under no tillage were collected in order to isolate the spores and multiply the AMF in pots. Alfa and beta diversity indexes were used. The spores were extracted from the soil by wet-sieving and centrifugation in sucrose, mounted on semi-permanent slides and identified. In areas I, II and III, 12, 17 and 15 AMF species, respectively, were verified. In the multiplication pots, six species were isolated in area I, 12 in area II and 10 in area III. It was concluded that the survey of AMF diversity is more efficient when the both techniques are associated.
Glomeromycota; no-tillage; taxonomy
