INTRODUCTION: The reliability of laboratory results is ensured by the implementation of quality control, which has basic functions, such as analysis, research and prevention of laboratory errors through programs that encompass both internal and external control. OBJECTIVE: To propose a standard method to use pooled whole blood as internal quality control in the Hematology division. METHOD: The selected whole blood samples were collected with EDTA, belonged to the same blood group and Rh factor and did not present interfering factors, such as hemolysis, lipemia and icterus. From a total of 30 ml of whole blood it was obtained 3 aliquots of 10 ml each, to which 0 ml (no addition), 1 ml and 5 ml of glycerol (preservative) were added, respectively. The samples were analyzed in the automatic counter ADVIA® 60. After the determination of mean and standard deviation values of all samples, they were evaluated for a period of 45 working days in order to produce a Levey-Jennings graph and to check their stability. RESULT AND CONCLUSION: We could verify that the pool of whole blood prepared in accordance with the methodology proposed does not present the required stability to be used as an alternative internal control in the Hematology division.
Quality control; Internal control; Pool; Hematology
