Storage of recalcitrant seeds of <i>Eugenia brasiliensis</i> Lam. under control of water availability

The reduction in humidity and temperature makes it possible to prolong the storage of seeds, except for those sensitive to desiccation (recalcitrant), which therefore cannot be included in ex situ conservation banks. One way to control the metabolic activity and the movement of water into or out of the seed is to use osmotically active chemicals, such as polyethylene glycol (PEG). In this study, the effect of storage of recalcitrant seeds of Eugenia brasiliensis with water movement control in osmotic medium was evaluated. The results showed that the maintenance of these seeds at water potentials between -1 and -2 MPa allowed increasing the capacity for conserving viability in storage. They also demonstrated that the expansion of this conservation, unlike what occurs with the reduction of temperature, is not necessarily associated with the reduction of metabolic rates.

Index terms:
deterioration; PEG; recalcitrance; tropical species

Authorship

Aline Testoni Cécel

Instituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil. Instituto de Pesquisas AmbientaisBrasilSão Paulo, SP, BrasilInstituto de Pesquisas Ambientais, Av. Miguel Stéfano 3687, São Paulo, SP 04301-902, Brasil.

https://orcid.org/0000-0003-3467-6968

Claudio José Barbedo ^**Corresponding author E-mail: cjbarbedo@yahoo.com.br

https://orcid.org/0000-0002-4417-7495

*Corresponding author
E-mail: cjbarbedo@yahoo.com.br

SCIMAGO INSTITUTIONS RANKINGS

Tables

Tables (7)

Thumbnail

Table 1
O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at four temperatures. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Thumbnail

Table 2
O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at 8 °C in airtight glass jars, loose or in paper rolls with water or PEG solution at -2 and -8 MPa. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Thumbnail

Table 3
Germination and formation of normal seedlings (Seedlings) of Eugenia brasiliensis seeds stored for 60 and 120 days at two temperatures (25 and 8 °C) and in perforated plastic bags (Plastic) or airtight glass jars (Glass). Means followed by the same letter (lowercase comparing packages, uppercase comparing temperatures), within each storage period, do not differ from each other (Tukey, 5%).

Thumbnail

Table 4
Water content (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 5
Water potential (MPa) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 6
Germination (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Thumbnail

Table 7
Production of normal seedlings (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Table 1 O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at four temperatures. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Temperature (°C)	O₂ consumption (μmol.gDM^-1.h^-1)	CO₂ release (μmol.gDM^-1.h^-1)	RQ
35	7.07 a	3.84 a	0.55 b
25	3.60 b	2.12 b	0.59 b
15	0.71 c	0.40 c	0.61 b
8	0.29 d	0.23 d	0.83 a

Table 2 O₂ consumption, CO₂ release and respiratory quotient (RQ) of Eugenia brasiliensis seeds, incubated at 8 °C in airtight glass jars, loose or in paper rolls with water or PEG solution at -2 and -8 MPa. Means followed by the same letter in the column do not differ from each other (Tukey, 5%).

Incubation	O₂ consumption (μmol.gDM^-1.h^-1)	CO₂ release (μmol.gDM^-1.h^-1)	RQ
Loose	0.25 a	0.16 a	0.71 b
Water	0.23 a	0.14 a	0.63 b
PEG -2 MPa	0.29 a	0.17 a	0.64 b
PEG -8 MPa	0.16 b	0.15 a	0.98 a

Table 3 Germination and formation of normal seedlings (Seedlings) of Eugenia brasiliensis seeds stored for 60 and 120 days at two temperatures (25 and 8 °C) and in perforated plastic bags (Plastic) or airtight glass jars (Glass). Means followed by the same letter (lowercase comparing packages, uppercase comparing temperatures), within each storage period, do not differ from each other (Tukey, 5%).

Storage (days)	Temperature (°C)	Package	Germination (%)	Seedlings (%)
60	25	Plastic	100 a A	93 a A
		Glass	0 b B	0 b B

	8	Plastic	90 a A	87 a A
		Glass	87 a A	77 a A

120	25	Plastic	100 a A	70 a B
		Glass	0 b B	0 b B

	8	Plastic	93 a A	93 a A
		Glass	87 a A	77 b A

Table 4 Water content (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	50.8	53.2	a	57.3	a	62.9	a	63.8	a
-1 MPa		50.4	ab	52.1	b	52.4	b	53.0	b
-2 MPa		49.6	b	50.1	b	51.1	b	51.1	b
-3 MPa		48.0	bc	45.7	c	47.4	c	47.1	c
-7 MPa		45.3	c	42.6	d	38.0	e	41.3	d
-8 MPa		45.0	c	40.9	d	42.0	d	42.4	d
-9 MPa		45.2	c	41.6	d	38.5	e	42.0	d

Table 5 Water potential (MPa) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	-0.81	-0.90	c	-1.05	f	-1.01	e	-0.80	d
-1 MPa		-1.32	bc	-0.89	f	-1.74	d	-1.55	c
-2 MPa		-0.96	c	-2.19	e	-2.46	c	-2.16	bc
-3 MPa		-1.74	ab	-3.21	d	-2.97	c	-2.70	b
-7 MPa		-2.25	a	-4.84	c	-4.12	b	-4.07	a
-8 MPa		-1.76	ab	-5.10	b	-4.23	ab	-4.04	a
-9 MPa		-2.15	a	-6.30	a	-4.89	a	-4.36	a

Table 6 Germination (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	100	100	a	30	c	0	c	0	c
-1 MPa		90	a	97	a	90	a	87	a
-2 MPa		97	a	100	a	90	a	80	a
-3 MPa		100	a	80	b	63	b	23	b
-7 MPa		40	b	0	d	0	c	0	c
-8 MPa		53	b	3	d	0	c	0	c
-9 MPa		13	c	0	d	0	c	0	c

Table 7 Production of normal seedlings (%) of Eugenia brasiliensis seeds without storage (0 days) or stored at 8 °C for 60, 180, 360 and 540 days in plastic bags, loose (Control) or in paper rolls with PEG solution of different water potentials (-1 to -9 MPa). Means followed by the same letter in the columns do not differ from each other (Tukey, 5%).

Water potential of the solution	Storage periods (days)
Water potential of the solution	Initial	60		180		360		540
Control	77	77	a	23	b	0	b	0	a
-1 MPa		70	a	70	a	53	a	13	a
-2 MPa		70	a	73	a	50	a	10	a
-3 MPa		77	a	60	a	30	a	7	a
-7 MPa		17	b	0	b	0	b	0	a
-8 MPa		30	b	0	b	0	b	0	a
-9 MPa		7	b	0	b	0	b	0	a

How to cite

copy

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Storage of recalcitrant seeds of <i>Eugenia brasiliensis</i> Lam. under control of water availability

PlumX

Mensagem

[1] *Corresponding author
E-mail: cjbarbedo@yahoo.com.br