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Validation of the Vigor-S image analysis system for the characterization of phytotoxicities in soybean seedlings

ABSTRACT:

Phytotoxic effects induced in soybean seedlings by exposure to various pesticides can lead to shortened and thickened hypocotyls, taproot atrophy, and reduced development or absence of secondary roots. This study aimed to validate the Vigor-S image analysis system for characterizing phytotoxic effects on soybean seedlings resulting from seed treatment using different products. Soybean seeds of cultivar BRS 284 received three seed treatments: cyproconazole fungicide, which causes hypocotyl shortening; glyphosate herbicide, which results in reduced root system; and control (without treatment). The experiment used a randomized complete block design with six replications. Seed physiological quality was assessed by the germination test, seedling length by the traditional method and also determined by the Vigor-S system method. There was a significant positive correlation when comparing seedling length parameters between the conventional and Vigor-S methods. It was concluded that seedlings exhibiting phytotoxicity symptoms from exposure to glyphosate herbicide and cyproconazole fungicide had reduced seedling length. The Vigor-S system effectively detects both types of phytotoxicity in soybean seedlings, which could have implications for agricultural practices.

Index terms:
computerized image analysis; Glycine max (L.) Merrill; seedling length

ABRATES - Associação Brasileira de Tecnologia de Sementes Av. Juscelino Kubitschek, 1400 - 3° Andar, sala 31 - Centro,, CEP 86020-000 Londrina/PR - Londrina - PR - Brazil
E-mail: jss@abrates.org.br