Delinting and neutralizers residue effect on stored cotton seeds physiological quality determined by phenotyping image analysis

The cottonseed delinting removes lint from the seed coat to improve plantability and maintain the seed’s physiological quality, therefore, this work aimed to determine the effect of delinting and neutralizers’ residual on the physiological quality of stored cottonseed by using image analysis phenotyping. The experiment was a randomized factorial design of 3 x 4 x 2 and two periods of storage evaluation (0 and 180 days). The cotton seeds were delinted at three different times (2, 4, and 10 minutes), neutralized using four distinct neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH), and there were seeds with and without neutralizer residue. The evaluations carried out were: water content, seed residual lint imaging, germination, seedling emergence, and seedling length imaging. The neutralizers Quicklime, Hydrated lime, and Filler lime do not need removal from the cotton seed coat. In storage, only the neutralizer NaOH has a high latent effect in lowering cottonseed quality, and the other neutralizers have lower physiological quality when the residue is removed. The image analysis phenotyping is efficient in identifying seedlings’ vigor.

Index terms:
Gossypium L.; imaging; lint; neutralization; vigor

Authorship

Juliana Maria Espíndola Lima ^**Corresponding author E-mail: espindolaj5@hotmail.com

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

https://orcid.org/0000-0003-3932-6051

Everson Reis Carvalho

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

https://orcid.org/0000-0003-4925-4100

Luiz Fernando de Souza Moraes

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

https://orcid.org/0000-0003-0487-3346

Nasma Henriqueta da Sorte Cossa

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

https://orcid.org/0000-0002-6279-6302

Felisberto Venâncio Chicamasso Miquicene

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

https://orcid.org/0000-0002-8998-0601

Yuri Fazion Gradela

KROM - Centro de Pesquisa e Inovação Industrial,171, 01311-000, São Paulo, SP, Brasil.KROMBrasilSão Paulo, SP, BrasilKROM - Centro de Pesquisa e Inovação Industrial,171, 01311-000, São Paulo, SP, Brasil.

https://orcid.org/0000-0001-9369-7748

*Corresponding author
E-mail: espindolaj5@hotmail.com

SCIMAGO INSTITUTIONS RANKINGS

Univerisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.Univerisdadee Federal de LavrasBrazilLavras, MG, BrazilUniverisdadee Federal de Lavras, 3037, 37200-900, Lavras, MG, Brasil.

Figures

Figures (6)

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Figure 1
Cotton seed residual lint imaging (%) after delinting (2, 4, and 10 minutes) and neutralization (with and without neutralizer residue). *Means followed by distinct letters in the column differ by the Scott-Knott test at p < 0.05. Coefficient of variation: 14.90%.

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Figure 2
Germination (%) of cottonseed submitted to different delinting times (2, 4, and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 4.55% (A) and 7.13% (B).

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Figure 3
Seedling emergence (%) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 3.19% (A) and 6.19% (B).

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Figure 4
Hypocotyl imaging (cm) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 7.15% (A) and 8.89% (B).

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Figure 5
Radicle imaging (cm) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 7.59% (A) and 7.56% (B).

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Figure 6
Phenotyping analysis of cottonseed seedling five days of germination after delinting (2, 4, and 10 minutes) and neutralization (with and without neutralizer residual) using different neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH) during storage 0 and 180 days. Source: Elaborated by the authors.

Figure 1 Cotton seed residual lint imaging (%) after delinting (2, 4, and 10 minutes) and neutralization (with and without neutralizer residue). *Means followed by distinct letters in the column differ by the Scott-Knott test at p < 0.05. Coefficient of variation: 14.90%.

Figure 2 Germination (%) of cottonseed submitted to different delinting times (2, 4, and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 4.55% (A) and 7.13% (B).

Figure 3 Seedling emergence (%) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 3.19% (A) and 6.19% (B).

Figure 4 Hypocotyl imaging (cm) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 7.15% (A) and 8.89% (B).

Figure 5 Radicle imaging (cm) of cottonseed submitted to different delinting times (2, 4 and 10 min), neutralizers (Quicklime, Hydrated lime, Filler lime and NaOH), neutralizer residue (with and without), and the storage period 0 (A) and 180 days (B). *Means followed by distinct lowercase letters differ in the same neutralizer residue and neutralizer between different delinting times; uppercase letters differ in the same neutralizer residue and delinting time between different neutralizers, and superscript letters differ in the same delinting time and neutralizer between neutralizer residue (Scott-Knott test at p < 0.05). Coefficient of variation: 7.59% (A) and 7.56% (B).

Figure 6 Phenotyping analysis of cottonseed seedling five days of germination after delinting (2, 4, and 10 minutes) and neutralization (with and without neutralizer residual) using different neutralizers (Quicklime, Hydrated lime, Filler lime, and NaOH) during storage 0 and 180 days. Source: Elaborated by the authors.

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Delinting and neutralizers residue effect on stored cotton seeds physiological quality determined by phenotyping image analysis

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[1] *Corresponding author
E-mail: espindolaj5@hotmail.com