In this paper we deal with the vertical distribution of the mosquitoes which we have been studying at the National Park of Serra dos Órgãos, state of Rio de Janeiro, Brazil. We have established, for a whole year, by means of concomitant weekly captures on human baits the preferences of the various species for feeding. A comparative analysis of the monthly vertical distribution is made, taking into account variations of temperature, humidity, rainfall and seasons of the year. Among the specimens with significant tendency for acrodendrophily we have found the following disease carriers: Anopheles cruzii - Humana and Simian Malarias; Culex nigripalpus - St. Louis Encephalitis (SLE); Haemagogus leucocelaenus and Ha. capricornii - Sylvan Yellow Fever, all for them also being present, though in smaller numbers, at the soil level. The sabethini: Wyemyia knabi, Phoniomyia theobaldi, Sabethes tarsopus, Sa. quasicyaneus and S. chloropterus complete the roll of the mains canopy feeders. On the other hand, Aedes fluviatilis, Trichoprosopon digitatus, Tr. fluviatilis, Tr. similis, Tr. frontosus, Tr. theobaldi, Wy. arthrostigma, Wy. aporonoma, Wy. mystes, Limatus durhami, L. pseudomesthisticus, Sa. identicus and Sa. undosus were captured normally, on baits close to the ground.