The Author investigated the antibacterial activity of 14 strains of Aspergillus niger from the National Collection of Type Cultures. In the Raulin & Mosseray medium, 7 strains presented total activity, never more than 1:10, against Staphylococcus aureus nº 553, The strains of the nrs. 1.161 and 2.390 remained active more than 40 days. The Czapek-Dox medium with 5% of corn-steep didi not improve the results obtained with the Raulin & Mosseray medium. In the yeast with peptone medium all the strains are inactive.