Abstract
The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
Leishmania; polymerase chain reaction; hybridization; molecular probes; mini-exon gene; minicircle; kDNA
Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
Win Degrave1
Octavio Fernandes2
David Campbell3
Marcelo Bozza4
Ulisses Lopes4
Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil
Universidade do Estado do Rio de Janeiro, Departamento de Patologia, Rio de Janeiro, Brasil
University of California, School of Medicine, Department of Microbiology and Immunology, Los Angeles, USA
Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Parasitologia Molecular, Rio de Janeiro, Brasil
The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
Full text available only in PDF format.
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Publication Dates
-
Publication in this collection
15 June 2009 -
Date of issue
Sept 1994
