Abstract
A flagellte trypanosomatid was isolated in culture from the digestive tract of the mosquito Culex saltanensis Dyar, 1928. It grows exuberantly in liver infusion in the form of epimastigotes and choanomastigotes typical of the genus Crithidia. The trypanosomatid was compared to C. deanei, C. fasciculata, C. luciliae, C. oncopelti and C. guilhermei. The techniques used for comparison were electron transmission microscopy, isoenzymes and kDNA restriction profiles. No endosymbionts were found at electron microscopy. Results for the biochemical methods employd indicate that the trypanosomatid isolated from C. saltanensis is a new species of Crithidia. The name C. ricardoi sp. n. is proposed for thes new species.
Crithidia ricardoi sp. n.; trypanosomatidae; Culex saltanensis; kDNA; isoenzymes; electron microscopy; cell sorting
Crithidia ricardoi sp. n. a new species of trypanosomatidae isolated from Culex saltanensis Dyar, 1928 (Diptera: Culicidae)
Alexander Sibajev1
Raquel da Silva Pacheco2
Maurílio J. Soares3
Elisa Cupolillo2
Alvaro Luiz Bertho dos Santos1
Hooman Momen2
Instituto Oswaldo Cruz, Departamento de Protozoologia, Rio de Janeiro, Brasil
Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil
Instituto Oswaldo Cruz, Departamento de Ultraestrutura e Biologia Celular, Rio de Janeiro, Brasil
A flagellte trypanosomatid was isolated in culture from the digestive tract of the mosquito Culex saltanensis Dyar, 1928. It grows exuberantly in liver infusion in the form of epimastigotes and choanomastigotes typical of the genus Crithidia. The trypanosomatid was compared to C. deanei, C. fasciculata, C. luciliae, C. oncopelti and C. guilhermei. The techniques used for comparison were electron transmission microscopy, isoenzymes and kDNA restriction profiles. No endosymbionts were found at electron microscopy. Results for the biochemical methods employd indicate that the trypanosomatid isolated from C. saltanensis is a new species of Crithidia. The name C. ricardoi sp. n. is proposed for thes new species.
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Publication Dates
-
Publication in this collection
01 June 2009 -
Date of issue
Dec 1993
