Morphometric studies on amastigotes of eigtheen populations from four isolates of two species of Venezuelan Leishmania (L. braziliensis and L. garnhami) indicate that the position of the kinetoplast is not significantly modified when parasites are submitted to pasages into hamsters, and culture in NNN medium or infection in a vector (Lu. townsendi). The location of the kinetoplast measured as the distance from the posterior end of the amastigote to the kinetoplast divided by the distance of the same organoid to the anterior end of the amastigote allows to differenciate L. braziliensis from L. mexicana and L. garnhami. Other morphometric parameters are not so confiable.