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Short Communication • Nauplius 24 • 2016 • https://doi.org/10.1590/2358-2936e2016030   copy

New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea)

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

We designed 14 new primers for amplification of the COI barcode region of decapod crustacean species. We tested, with high level of success, the generation of ~ 640 ± 49 base-pair sequences in selected groups of decapods (hermit crabs, squat lobsters, marine and freshwater crabs and shrimps), encompassing representatives of 27 genera of 15 families, 11 of Pleocyemata (Anomura, Brachyura, and Caridea) and 4 of Dendrobranchiata. Based on the results we expect the applicability of these primers for several studies with different taxa within Decapoda.

Key words:
COI; DNA Barcoding; molecular markers; molecular techniques

Sociedade Brasileira de Carcinologia Instituto de Biociências, UNESP, Campus Botucatu, Rua Professor Doutor Antônio Celso Wagner Zanin, 250 , Botucatu, SP, 18618-689 - Botucatu - SP - Brazil
E-mail: editor.nauplius@gmail.com
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