Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

Piscor, Diovani; Fernandes, Carlos Alexandre; Parise-Maltempi, Patricia P.

doi:10.1590/1982-0224-20170066

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Original article • Neotrop. ichthyol. 16 (1) • 2018 • https://doi.org/10.1590/1982-0224-20170066 copy

Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae)

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ABSTRACT

The chromosomal location of 5S rRNA and U2 snRNA genes of Piabina argentea, Piabarchus stramineus and two Bryconamericus species from two different Brazilian river basins were investigated, in order to contribute to the understanding of evolutionary characteristics of these repetitive DNAs in the subfamily Stevardiinae. The diploid chromosome number was 2n = 52 for Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus and Piabina argentea. The 5S rDNA clusters were located on one chromosome pair in P. stramineus and B. cf. iheringii, and on two pairs in B. turiuba and P. argentea. The U2 snDNA clusters were located on the one pair in all species. Two-color FISH experiments showed that the co-localization between 5S rDNA and U2 snDNA in P. stramineus can represent a marker for this species. Thus, the present study demonstrated that the number of U2 snDNA clusters observed for the four species was conserved, but particular characteristics can be found in the genome of each species.

Keywords:
Repetitive DNA; Splicing; 5S rDNA; Chromosome; Diploid number

Genera/Species	Localities/States	2n^b	18S^d	5S^e	U2^f	References
Bryconamericus
B. aff. exodon	Três Bocas Stream (PR)	52	8	−	−	Paintner-Marques et al. (2002Paintner-Marques TR, Giuliano-Caetano L, Dias AL. Multiple NORs in Bryconamericus aff. exodon (Osteichthyes, Characidae, Tetragonopterinae). Hereditas . 2002; 137(2):107-12.)
B. aff. iheringii	Água Floresta River (PR)	52	2	−	−	Paintner-Marques et al. (2003Paintner-Marques TR, Giuliano-Caetano L, Dias AL. Cytogenetic characterization of a population of Bryconamericus aff. iheringii (Characidae, Tetragonopterinae). Genet Mol Biol . 2003; 26(2):145-49.)
B. aff. iheringii cyt-I^a	Maringá Stream (PR)	52	6	−	−	Capistano et al. (2008Capistano TG, Portela-Castro ALB, Júlio HF Jr. Chromosome divergence and NOR polymorphism in Bryconamericus aff. iheringii (Teleostei, Characidae) in the hydrographic systems of the Paranapanema and Ivaí Rivers, Paraná, Brazil. Genet Mol Biol. 2008; 31(1):203-07.)
B. aff. iheringii cyt-II^a	Keller River (PR)	52	10	−	−
B. aff. iheringii cyt-III^a	Tatupeba Stream (PR)	52	2	−	−
B. ecai cyt-I^a	Forquetinha River (RS)	52	4	−	−	Santos et al. (2012Santos AR, Rubert M, Giuliano-Caetano L, Dias AL. Sympatric occurrence of four cytotypes and one extra chromosome in Bryconamericus ecai (Characidae): 18S rDNA polymorphism and heterochromatin composition. Hereditas . 2012; 149(1):24-33.)
B. ecai cyt-II^a	Forquetinha River (RS)	52	2	−	−
B. ecai cyt-III^a	Forquetinha River (RS)	52 + B^c	6	−	−
B. ecai cyt-IV^a	Forquetinha River (RS)	52	2	−	−
B. turiuba	Tributary of Passa-Cinco River (SP)	52	4	4	−	Piscor et al. (2013Piscor D, Ribacinko-Piscor DB, Fernandes CA, Parise-Maltempi PP. Cytogenetic analysis in three Bryconamericus species (Characiformes, Characidae): first description of the 5S rDNA-bearing chromosome pairs in the genus. Mol Cytogenet [serial on internet]. 2013; 6(13):1-8. Available from: https://doi.org/10.1186/1755-8166-6-13 https://doi.org/10.1186/1755-8166-6-13... )
B. cf. iheringii	Tributary of Corumbataí River (SP)	52	2	2	−
B. aff. iheringii cyt-I^a	Três Bocas Stream (PR)	52	2	−	−	Silva et al. (2014Silva LLL, Giuliano-Caetano L, Dias AL. Karyotypic diversity in a population of Bryconamericus aff. iheringii (Characidae). Genet Mol Res. 2014; 13(1):2069-81.)
B. aff. iheringii cyt-II^a	Três Bocas Stream (PR)	52	8	−	−
B. aff. iheringii cyt-III^a	Três Bocas Stream (PR)	52	6	−	−
B. aff. iheringii cyt-IV^a	Três Bocas Stream (PR)	52	6	−	−
B. aff. iheringii cyt-V^a	Três Bocas Stream (PR)	52	8	−	−
B. aff. iheringii cyt-VI^a	Três Bocas Stream (PR)	52	8	−	−
B. aff. iheringii	Ocoí River (PR)	52	2	−	−	Nishiyama et al. (2015Nishiyama PB, Rossi MMV, Porto FE, Borin LA, Portela-Castro ALB, Martins-Santos IC. Cytogenetic studies in species of stream fishes: Hyphessobrycon vinaceus, Bryconamericus aff. iheringii and Odontostilbe pequira (Pisces: Characidae). Evolução e Conservação da Biodiversidade. 2015; 6(1):13-22.)
B. ecai cyt-V^a	Forquetinha River (RS)	52	4	6	2	Santos et al. (2017Santos ARD, Usso MC, Gouveia JG, Araya-Jaime C, Frantine-Silva W, Giuliano-Caetano L, Foresti F, Dias AL. Chromosomal mapping of repetitive DNA sequences in the genus Bryconamericus (Characidae) and DNA barcoding to differentiate populations. Zebrafish [serial on internet]. 2017; 14(3):261-71. Available from: https://doi.org/10.1089/zeb.2016.1380 https://doi.org/10.1089/zeb.2016.1380... )
B. ecai cyt-VI^a	Forquetinha River (RS)	52	13	8	2
B. ecai cyt-VII^a	Forquetinha River (RS)	52	10	7	2
Bryconamericus sp. (Group 1)	Vermelho River (PR)	52	4	6	2
Bryconamericus sp. (Group 2)	Vermelho River (PR)	52	16	8	2
Bryconamericus sp. (Cambuta)	Cambuta River (PR)	52	6	2	2
B. turiuba	Tributary of Passa-Cinco River (SP)	52	−	4	2	Present study
B. cf. iheringii	Tributary of Corumbataí River (SP)	52	−	2	2	Present study
Piabarchus stramineus
P. stramineus	Guaçu Stream (MS)	52	2	2	−	Piscor et al. (2013Piscor D, Ribacinko-Piscor DB, Fernandes CA, Parise-Maltempi PP. Cytogenetic analysis in three Bryconamericus species (Characiformes, Characidae): first description of the 5S rDNA-bearing chromosome pairs in the genus. Mol Cytogenet [serial on internet]. 2013; 6(13):1-8. Available from: https://doi.org/10.1186/1755-8166-6-13 https://doi.org/10.1186/1755-8166-6-13... )
P. stramineus	Guaçu Stream (MS)	52	−	2	2
Piabina
P. argentea	São Francisco River (MG)	52	6	4	−	*Peres et al. (2008)*Peres WAM., WAM, Bertollo LAC, Moreira-Filho O. Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes). Genet Mol Biol . 2008; 31(1):222-26.
P. argentea	Municipality of Itatinga (SP)	52	2	4	−	Pazian et al. (2012Pazian MF, Pereira LHG, Shimabukuru-Dias CK, Oliveira C, Foresti F. Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae). Neotrop Ichthyol. 2012; 10(2):329-40.)
P. argentea	Municipality of Botucatu (SP)	52	4	4	−
P. argentea	Municipality of Bauru (SP)	52	6	6	−
P. anhembi	Municipality of Salesópolis (SP)	52	2	2	−
P. argentea	Tributary of Passa-Cinco River (SP)	52	−	4	2	Present study

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