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Notas Científicas • Pesq. agropec. bras. 46 (7) • July 2011 • https://doi.org/10.1590/S0100-204X2011000700014 linkcopy

Open-access Identification of the begomovirus resistance genes Ty-2 and Ty-3 in tomato genotypes

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    The objective of this work was to identify the presence of the genes begomovirus resistence Ty-2 and Ty-3 in tomato accessions of the Banco de Germoplasma de Hortaliças of the Universidade Federal de Viçosa. The oligonucleotides TO302 F/R and FLUW25 F/R were used in PCR analysis to verify the presence of markers associated to the genes Ty-2 and Ty-3, respectively. The Ty-2 gene was observed in heterozygosis in the accession BGH-6881 (Solanum peruvianum), and the Ty-3 gene in homozygosis in the accessions BGH-6878, BGH-6897 (S. lycopersicum), and in heterozygosis in acession BGH-6881. The identification of resistance genes, using PCR analysis, represents an advance for tomato breeding programs in Brazil.

    Solanum lycopersicum; Solanum peruvianum; geminivirus; PCR; genetic resources

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