In vitro organogenesis as an efficient method for the propagation of <i>Dalbergia nigra</i>

The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L^-1 BAP show the best combination for the in vitro propagation of D. nigra.

Index terms:
Dalbergia nigra ; Brazilian rosewood; explants; in vitro culture; 6-benzylaminopurine; thidiazuron

Authorship

Ingridh Medeiros Simões Corresponding author

Universidade Federal do Espírito Santo, Centro de Ciências Agrárias e Engenharias, Departamento de Ciências Florestais e da Madeira, Avenida Governador Lindemberg, nº 316, Centro, CEP 29550-000 Jerônimo Monteiro, ES, Brazil. E-mail: simoes.ingridh@gmail.com, carolinepalacio@yahoo.com.br, tamyrisdemello@gmail.com, thuannyer@gmail.com, natashav.oliveira@gmail.com, mvwcaldeira@gmail.com, rodrigosobreiraalexandre@gmail.comUniversidade Federal do Espírito SantoBrazilJerônimo Monteiro, ES, BrazilUniversidade Federal do Espírito Santo, Centro de Ciências Agrárias e Engenharias, Departamento de Ciências Florestais e da Madeira, Avenida Governador Lindemberg, nº 316, Centro, CEP 29550-000 Jerônimo Monteiro, ES, Brazil. E-mail: simoes.ingridh@gmail.com, carolinepalacio@yahoo.com.br, tamyrisdemello@gmail.com, thuannyer@gmail.com, natashav.oliveira@gmail.com, mvwcaldeira@gmail.com, rodrigosobreiraalexandre@gmail.com

http://orcid.org/0000-0002-7483-9349

Caroline Palacio de Araujo Corresponding author

http://orcid.org/0000-0002-0259-2543

Tamyris de Mello Corresponding author

http://orcid.org/0000-0003-1189-5404

Thuanny Lins Monteiro Rosa Corresponding author

http://orcid.org/0000-0001-6979-6914

Natasha Vieira de Oliveira Corresponding author

http://orcid.org/0000-0001-6376-9544

Marcos Vinícius Winckler Caldeira Corresponding author

http://orcid.org/0000-0003-4691-9891

Edilson Romais Schmildt Corresponding author

Universidade Federal do Espírito Santo, Centro Universitário Norte do Espírito Santo, Departamento de Ciências Agrárias e Biológicas, Rodovia BR-101, Km 60, CEP 29932-540 São Mateus, ES, Brazil. E-mail: e.romais.s@gmail.comUniversidade Federal do Espírito SantoBrazilSão Mateus, ES, BrazilUniversidade Federal do Espírito Santo, Centro Universitário Norte do Espírito Santo, Departamento de Ciências Agrárias e Biológicas, Rodovia BR-101, Km 60, CEP 29932-540 São Mateus, ES, Brazil. E-mail: e.romais.s@gmail.com

http://orcid.org/0000-0002-5248-6773

José Carlos Lopes Corresponding author

Universidade Federal do Espírito Santo, Centro de Ciências Agrárias e Engenharias, Departamento de Produção Vegetal, Alto Universitário, s/nº, Guararema, CEP 29500-000 Alegre, ES, Brazil. E-mail: jcufes@bol.com.brUniversidade Federal do Espírito SantoBrazilAlegre, ES, BrazilUniversidade Federal do Espírito Santo, Centro de Ciências Agrárias e Engenharias, Departamento de Produção Vegetal, Alto Universitário, s/nº, Guararema, CEP 29500-000 Alegre, ES, Brazil. E-mail: jcufes@bol.com.br

http://orcid.org/0000-0002-3457-7997

Wagner Campos Otoni Corresponding author

Universidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde, Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos Vegetais/ Bioagro, Campus Universitário, Avenida Peter Henry Rolfs, s/nº, CEP 36570-900 Viçosa, MG, Brazil. E-mail: wcotoni@gmail.comUniversidade Federal de ViçosaBrazilViçosa, MG, BrazilUniversidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde, Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos Vegetais/ Bioagro, Campus Universitário, Avenida Peter Henry Rolfs, s/nº, CEP 36570-900 Viçosa, MG, Brazil. E-mail: wcotoni@gmail.com

http://orcid.org/0000-0002-4880-0547

Rodrigo Sobreira Alexandre Corresponding author

http://orcid.org/0000-0002-9614-9373

Corresponding author

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (2)

Thumbnail

Figure 1
Schematic representation of Dalberia nigra morphology and experimental setup: A and B, branch with fruit and seed; C, in vitro germination in woody plant medium (WPM) leading to the formation of a normal seedling; D–G, sectioning of explants from a cotyledonary node (D, E), or from a node segment (F, G). Illustration by Caroline Palacio de Araujo

Thumbnail

Figure 2
In vitro morphogenesis of juvenile explants of D. nigra: A, (a) number of shoots, (b) shoot length (cm), (c) number of leaves, (d) shoot dry mass (mg), (e) callus diameter (mm), (f) number of roots, (g) root length (cm), and (h) root dry mass (mg). ^(¹) Means followed by equal lowercase letters between cytokinin combinations within each explant do not differ from each other, by the Tukey’s test, at 5% probability. *Significant between the two types of explants, by the he F-test, at 5% probability. ^nsNonsignificant. B, Pearson’s correlation between the different morphological variables, for explants from cotyledonary nodes (a) and nodal segments (a), for the following parameters: NS, number of shoots; SL, shoot length; NL, number of leaves; SDM, shoot dry mass; CD, callus diameter; NR, number of roots; RL, root length; and RDM, root dry mass. Correlation values greater than 0.48 are significant, by the t-test, at 1% probability.

Figure 1 Schematic representation of Dalberia nigra morphology and experimental setup: A and B, branch with fruit and seed; C, in vitro germination in woody plant medium (WPM) leading to the formation of a normal seedling; D–G, sectioning of explants from a cotyledonary node (D, E), or from a node segment (F, G). Illustration by Caroline Palacio de Araujo

Figure 2 In vitro morphogenesis of juvenile explants of D. nigra: A, (a) number of shoots, (b) shoot length (cm), (c) number of leaves, (d) shoot dry mass (mg), (e) callus diameter (mm), (f) number of roots, (g) root length (cm), and (h) root dry mass (mg). ^(¹) Means followed by equal lowercase letters between cytokinin combinations within each explant do not differ from each other, by the Tukey’s test, at 5% probability. *Significant between the two types of explants, by the he F-test, at 5% probability. ^nsNonsignificant. B, Pearson’s correlation between the different morphological variables, for explants from cotyledonary nodes (a) and nodal segments (a), for the following parameters: NS, number of shoots; SL, shoot length; NL, number of leaves; SDM, shoot dry mass; CD, callus diameter; NR, number of roots; RL, root length; and RDM, root dry mass. Correlation values greater than 0.48 are significant, by the t-test, at 1% probability.

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In vitro organogenesis as an efficient method for the propagation of <i>Dalbergia nigra</i>

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[1] Corresponding author