Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

Criopreservação de embriões zigóticos de coqueiro Anão Verde do Brasil de Jiqui

Abstract:

The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L^-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L^-1 sucrose + 1 mg L^-1 gibberellic acid + 1 g L^-1 activated charcoal + 2.2 g L^-1 Gelrite; and M2, Y3 medium + 60 g L^-1 sucrose + 2.2 g L^-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.

Index terms:
Cocos nucifera; ex situ conservation; PVS3