During 150 days 12 female Quarter Horse yearlings at one year of age were kept solely on Brachiaria humidicola pasture, on a complete randomized design, with three treatments and four replications: level 0 (NRC1989); level 50 (50% above NRC); level 100 (100% above NRC). It was used 270 g of sugar as an intake promoter. Biopsis were perfomed on the wing of ileum of each animal, one at the onset and the other at the end of trial, in order to evaluate Ca mobilization and Ca:P of the bone. Forage samples were collected monthly to evaluate oxalate levels and chemical composition. There was a time effect on Ca mobilization and Ca:P (P< 0.05), regardless the treatments. The average of the three treatments, at the onset and at the end of the trial, for Ca and P levels and Ca:P was, respectively: 10.05, 5.22, 1.93:1 and 6.24, 4.06, 1.54:1. The levels of oxalate in the grass varied from 1.18 to 2.00%. Suplementing Ca to young horses at a level 200% greater than the National Research Council recomendation did not prevent Ca mobilization from the bone when the animals were grazing Brachiaria humidicola for an extended period of time.
bone biopsis; oxalate
