<i>In vitro</i> shoot regeneration in <i>Myracrodruon urundeuva</i> Fr. All

Figure 1
Number of shoots (A) and shoot length (B), at 45 days after inoculation. Values obtained from the basal nodal segment, medial nodal segment and apical segment of Myracrodruon urundeuva Fr. All. under concentrations of meta-topolin (mT).^ns, * and **: not significant and significant at 5 % and 1 % of probability, respectively, by the F test.

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Figure 2
Myracrodruon urundeuva Fr. All. shoots at 45 days after inoculation. Values obtained from explants under concentrations of meta-topolin (mT). A) 16.0 µM of mT (medial segment); B) 2.0 µM of mT (basal segment); C) 16.0 µM of mT (basal segment); D) 32.0 µM of mT (basal segment).

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Table 1
Summary of the analysis of variance for percentage of explants responsive to shoot regeneration (%RE), number of shoots (NS) and shoot length (SL), at 45 days after inoculation. Values obtained from the hypocotyl and cotyledonary node explants (EXP) of Myracrodruon urundeuva Fr. All. under concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA).

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Table 2
Percentage of explants responsive to shoot regeneration (%RE), number of shoots (NS) and shoot length (SL), at 45 days after inoculation. Values obtained from the hypocotyl (HYP) and cotyledonary node (CN) explants of Myracrodruon urundeuva Fr. All. under concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA).

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Table 3
Summary of the analysis of variance for percentage of explants responsive to shoot regeneration (%RE), percentage of callus explants (%CE), number of shoots (NS) and shoot length (SL), at 45 days after inoculation. Values obtained from the basal nodal segment (first pair of leaves; biaxillary), medial nodal segment (uniaxillary) and apical segment explants (EXP) of Myracrodruon urundeuva Fr. All. under concentrations of meta-topolin (mT).

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Table 4
Means for percentage of explants responsive to shoot regeneration (%RE) and percentage of callus explants (%CE), at 45 days after inoculation. Values obtained from basal nodal, medial nodal and apical segment explants of Myracrodruon urundeuva Fr. All. under concentrations of meta-topolin (mT).

Source of variation	DF	Mean square
Source of variation	DF	%RE^x x Data transformed into arcsine √%.	NS^z z Data transformed by the function (x + 1)0.5.	SL (mm)^z z Data transformed by the function (x + 1)0.5.
BAP	4	0.42^ ,	0.08^ ,	1.27^ ,
NAA	2	0.91^ ,	0.27^ ,	2.05^ ,
EXP	1	2.20^ ,	0.89^ ,	5.60^ ,
BAP x NAA	8	0.49^ ,	0.15^ ,	1.09^ ,
BAP x EXP	4	0.13^ns ns significant at 1 % and 5 % of probability and not significant, respectively, by the F test.	0.04^ns ns significant at 1 % and 5 % of probability and not significant, respectively, by the F test.	0.20^ns ns significant at 1 % and 5 % of probability and not significant, respectively, by the F test.
NAA x EXP	2	1.07^ ,	0.26^ ,	1.98^ ,
BAP x NAA x EXP	8	0.69^ ,	0.20^ ,	1.61^ ,
Residue	150	0.09	0.02	0.27
CV (%)		210.11	14.20	41.97

BAP (µM)	^{___________________} %RE^{___________________}		^{___________________} NS^{___________________}		^{_____________} SL (mm)^{_____________}
BAP (µM)	HYP	CN	HYP	CN	HYP	CN
0.0 µM of NAA
0.0	0.00 aA	13.33 aAB	0.00 aA	0.20 aAB	0.00 aA	1.50 aA
2.0	36.67 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	13.33 aAB	0.37 aA	0.13 aB	4.33 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	2.20 aA
4.0	0.00 aA	0.00 aB	0.00 aA	0.00 aB	0.00 aA	0.00 aA
8.0	0.00 aA	0.00 aB	0.00 aA	0.43 aAB	0.00 aA	1.17 aA
16.0	0.00 bA	46.67 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	1.03 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	3.37 aA
1.5 µM of NAA
0.0	0.00 aA	6.67 aA	0.00 aA	0.07 aA	0.00 aA	0.53 aA
2.0	0.00 aA	0.00 aA	0.00 aA	0.00 aA	0.00 aA	0.00 aA
4.0	0.00 aA	0.00 aA	0.00 aA	0.00 aA	0.00 aA	0.00 aA
8.0	0.00 aA	6.67 aA	0.00 aA	0.13 aA	0.00 aA	0.17 aA
16.0	0.00 aA	10.00 aA	0.00 aA	0.17 aA	0.00 aA	0.30 aA
3.0 µM of NAA
0.0	0.00 aA	0.00 aB	0.00 aA	0.00 aB	0.00 aA	0.00 aB
2.0	0.00 bA	76.67 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	1.97 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	8.67 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.
4.0	0.00 bA	76.67 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	1.63 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.	0.00 bA	6.73 aA^* * differ from the others among NAA concentrations for the respective BAP and the same type of explant by the Tukey test at 5 % of probability.
8.0	0.00 bA	26.67 aB	0.00 aA	0.43 aB	0.00 aA	0.97 aB
16.0	3.33 aA	0.00 aB	0.07 aA	0.00 aB	0.10 aA	0.00 aB

Source of variation	DF	Mean square
Source of variation	DF	%RE^x x Data transformed into arcsine √%.	NS^z z Data transformed by the function (x + 1)0.5.	SL(mm)	%CE^x x Data transformed into arcsine √%.
mT	5	0.231^ns ns significant at 1 % and 5 % of probability and not significant, respectively, by the F test.	0.171^ ,	48.373^ ,	1.057^ ,
EXP	2	2.984^ ,	0.063^ns ns significant at 1 % and 5 % of probability and not significant, respectively, by the F test.	247.034^ ,	0.993^ ,
mT x EXP.	10	0.506^ ,	0.095^ ,	40.880^ ,	0.281^* * and
Residue	90	0.149	0.022	13.257	0.093
CV (%)		42.02	10.60	43.23	77.09

mT (µM)	Explant type
mT (µM)	Basal segment	Medial segment	Apical segment
%RE
0.0	10.00 cB	55.83 bA	92.50 aA
2.0	76.39 abA	55.00 bA	87.50 aA
4.0	56.66 bA	70.00 abA	93.33 aA
8.0	47.22 bA	62.50 bA	94.44 aA
16.0	71.67 bA	83.33 aA	74.17 aA
32.0	73.33 aA	79.17 aA	82.50 aA
%CE
0.0	3.33 aC	10.83 aC	10.83 aA
2.0	55.56 aAB	65.83 aAB	20.83 bA
4.0	38.33 aBC	49.17 aABC	35.03 aA
8.0	13.89 aC	29.17 aBC	30.56 aA
16.0	63.33 aAB	46.67 abABC	19.17 bA
32.0	80.00 aA	75.00 aA	25.83 bA

[TFN1] ^**
,

[TFN2] ^*
and

[TFN3] ^ns
significant at 1 % and 5 % of probability and not significant, respectively, by the F test.

[TFN4] ^z
Data transformed by the function (x + 1)^0.5.

[TFN5] ^x
Data transformed into arcsine √%.

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Brasil

In vitro shoot regeneration in Myracrodruon urundeuva Fr. All

Regeneração in vitro de brotações em Myracrodruon urundeuva Fr. All.

ABSTRACT