Protein Profiles of Individuals with Erosive Tooth Wear

Three types of saliva sampling were performed to obtain saliva from 34 18-year old individuals that received regular dental check-ups, along with clinical status of the dentition and risk factor related to erosive tooth wear using the VEDE scale. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample to test for association with erosive tooth wear status.

Results:

There were no differences in the number of detectable proteins sourced from the parotid gland, nor the unstimulated and stimulated whole saliva. Five out of the 34 individuals had no signs of erosive tooth wear despite an acidic diet and were more likely to have proteins with molecular weight smaller than 1 KDa (p=0.03).

Conclusion:

There is potential for the use of protein profiling to determine risks for erosive tooth wear.

Keywords:
Dental Enamel; Dental Caries; Tooth Erosion

Participant	Sex	Erosive Tooth Wear	Caries Experience	Number of Bands in Gel
			(DMFT score)	Parotid Saliva	Unstimulated Saliva	Stimulated Saliva
1	Female	No Erosion	12	6	8	8
2	Male	Dentin Exposed	5	7	9	7
3	Male	No Erosion	2	6	7	7
4	Male	Dentin Exposed	2	9	7	6
5	Female	No Erosion	0	6	9	8
6	Female	No Erosion	4	6	12	7
7	Female	Dentin Exposed	7	6	8	11
8	Female	Enamel Affected Only	5	10	7	6
9	Male	Enamel Affected Only	7	8	10	6
10	Male	Dentin Exposed	2	16	10	9
11	Male	Dentin Exposed	3	10	11	7
12	Male	Dentin Exposed	2	8	16	10
13	Female	Dentin Exposed	0	6	9	7
14	Male	Dentin Exposed	0	6	13	10
15	Male	Dentin Exposed	5	8	11	12
16	Male	Dentin Exposed	1	11	10	9
17	Female	Dentin Exposed	0	9	8	12
18	Male	Dentin Exposed	6	7	4	7
19	Male	Dentin Exposed	3	9	6	7
20	Male	Dentin Exposed	5	6	10	13
21	Female	Enamel Affected Only	7	11	12	14
22	Female	Enamel Affected Only	7	9	8	7
23	Male	Dentin Exposed	3	10	6	14
24	Male	Dentin Exposed	0	8	14	11
25	Female	Enamel Affected Only	0	7	11	6
26	Male	Enamel Affected Only	3	6	6	8
27	Male	Dentin Exposed	9	7	11	6
28	Male	Dentin Exposed	3	9	11	11
29	Female	Enamel Affected Only	8	6	8	10
30	Female	No Erosion	2	7	10	7
31	Female	Dentin Exposed	4	8	11	10
32	Female	Enamel Affected Only	11	7	11	6
33	Female	Enamel Affected Only	21	7	12	10
34	Female	Enamel Affected Only	7	9	14	11

Proteins Patterns	Individual Samples^* * According to individual identification in Table 1.
Presence of proteins with molecular weight above 260 KDa	Parotid saliva of 16, 22, and 23; stimulated saliva of 23
Absent agglutinin	Parotid saliva of 21 and 28; unstimulated saliva of 10
Reduced agglutinin	Parotid saliva of 2, 8, 11, 18, and 25; unstimulated saliva of 4, 28, and 33; stimulated saliva of 4 and 10
Reduced histatin	Parotid saliva of 2, 18, 19, and 28; unstimulated saliva of 18
Presence of proteins with molecular weight smaller than 1KDa	Parotid saliva of 7, 8, 9, 12, and 14; unstimulated saliva of 1, 3, 5, 7, 8, 13, 14, and 24; stimulated saliva of 3, 6, 7, 11, and 12

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[1] Author to whom correspondence should be addressed: Alexandre R. Vieira, Department of Oral Biology, 412 Salk Pavilion, University of Pittsburgh Schoolof Dental Medicine, Pittsburgh, PA, USA. 15261. Phone: +1 412-383-8972. E-mail: arv11@pitt.edu.