Abstract
Objective:
To study the adherence of Streptococcus mutans biofilm after induction with sucrose and xylitol.
Material and Methods:
Laboratory experimental study incorporating posttest-only control group design. S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol. An adherence assay was conducted in accordance with the method applied previously. The quantity of adhered bacteria was measured by means of a spectrophotometer at 570 nm. The data were presented as mean and standard deviation.
Results:
A biofilm induced with sucrose has a higher adherence level (0.9294 ± 0.0431) compared with one induced with xylitol (0.5095 ± 0.0392). Sucrose induces adherence levels by increasing glucan binding protein and glucosyltransferase of the bacteria, whereas xylitol will inhibit the glycolysis process of the bacteria.
Conclusion:
The adherence of sucrose-induced S. mutans biofilm is higher than that of xylitol-induced S. mutans biofilm.
Keywords:
Dental Plaque; Streptococcus mutans; Sucrose; Xylitol