The objective of this study was to select sugarcane genotypes through PROTOX inhibitors and antioxidants to induce the accumulation of protoporphyrin IX (PROTO IX), or its precursors in plants. Such compounds can be used as sensitizing agents in photodynamic therapy (PDT), being considered a low cost source for the treatment of malignancies. The experiment was set up on a temperature-controlled chamber, with 9 treatments being applied: (1. oxyfluorfen + monosodium glutamate + vitamin C and E; 2. oxyfluorfen + monosodium glutamate + vitamin C and E + levulenic acid; 3. oxyfluorfen; 4. carfentrazone + monosodium glutamate + vitamin C and E; 5. carfentrazone + monosodium glutamate + vitamin C and E + levulenic acid; 6. carfentrazone; 7. control + vitamin C and E; 8. control + vitamin C and E + levulenic acid; 9. control without spraying in 8 sugarcane genotypes (PO933499, RB806043, RB470355, PO830698, SP701143, PO901387, PO894414 and 8.SP903414); arranged in a factorial 9 x 8, with 4 repetitions. The repetitions were constituted by specific leaves (20 cm) from each genotype, which were sprayed with the appropriate treatments in a stationary simulator. Visual evaluations of the control were carried out at 2 DAA (Days After Application), and at the end of the study, analytical determinations were conducted via fresh biomass extraction to verify the protoporphyrin IX contents by high performance liquid chromatography. The results showed that significant short-term increases were observed in the PROTO IX concentrations for the genotypes RB470355, SP903414 submitted to treatment 2 and for genotype SP701143 submitted to treatment 8, indicating that they can be used as accumulating sources of protoporphyrin IX.
glutamate; protoporphyrin IX; sugarcane
