GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF <i>Chenopodium album</i> AGAINST <i>Sclerotium rolfsii</i>

ALI, A.; JAVAID, A.; SHOAIB, A.

doi:10.1590/S0100-83582017350100046

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Article • Planta daninha 35 • 2017 • https://doi.org/10.1590/S0100-83582017350100046 copy

GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF Chenopodium album AGAINST Sclerotium rolfsii

Análise por Cromatografia Gasosa com Espectrômetro de Massa (GC-MS) e Atividade Antifúngica de Extrato Metanólico de Raiz de Chenopodium album Diante de Sclerotium rolfsii

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Sclerotium rolfsii is a soil-borne fungal plant pathogen that causes diseases in more than 500 plant species. Chemical fungicides used to control this disease cause environmental pollution, therefore, plant derived compounds can be used as alternative to synthetic fungicides to reduce environmental pollution. Chenopodium album is a weed of family Chenopodiaceae that is used as food and also has medicinal importance. In the present study, antifungal activity of methanolic root extract of C. album was evaluated against S. rolfsii using six concentrations viz. 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 g 100 mL-1 amended in malt extract as growth medium. All the root extract concentrations significantly reduced fungal biomass by 15-58% over control. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic root extract of C. album was performed. Six compounds were identified in methanolic root extract through GC-MS analysis. The most abundant compound was 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (58.56%) followed by 9-octadecenoic acid (Z)-, methyl ester (12.75%) and 9-octadecenoic acid (Z)-, methyl ester (10.27%), which might be responsible for antifungal activity of methanolic root extract of C. album.

Keywords:
biological control; natural compounds; reduction use of agrochemical

Sociedade Brasileira da Ciência das Plantas Daninhas Departamento de Fitotecnia - DFT, Universidade Federal de Viçosa - UFV, 36570-000 - Viçosa-MG - Brasil, Tel./Fax::(+55 31) 3899-2611 - Viçosa - MG - Brazil
E-mail: rpdaninha@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Corresponding autor: <arshadjpk@yahoo.com>

Comp. No.	Names of compounds	Molecular formula	Molecular weight	Retention time (min)	Peak area (%)
1	2(3H)-Furanone, dihydro-4,4-dimethyl	C6H10O2	114	9.881	12.75
2	6-Methylenebicyclo(3.2.0)hept-3-en-2-one	C₈H₈O	120	10.773	7.21
3	Hexadecanoic acid, methyl ester	C17H34O2	270	18.716	5.44
4	9,12-Octadecadienoic acid (Z,Z)-, methyl ester	C19H34O2	294	20.355	5.81
5	9-Octadecenoic acid (Z)-, methyl ester	C19H36O2	296	20.406	10.27
6	1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl) ester	C16H22O4	278	24.238	58.50

Comp . No.	Names of compounds	Nature	Property	Reference
1	2(3H)-Furanone, dihydro-4,4-dimethyl	Ketone	No activity reported
2	6-Methylenebicyclo(3.2.0)hept-3-en-2-one	ketone	Bacteriostatic, fungistatic, antiparasitic	Hussein et al. (2016Hussein H.J., Hadi M.Y., Hameed I.H. Study of chemical composition of Foeniculum vulgare using Fourier transform infrared spectrophotometer and gas chromatography - mass spectrometry. J Pharm Phytother. 2016;8:60-89.)
3	Hexadecanoic acid, methyl ester	Palmitic acid methyl ester (fatty acid methyl ester)	Antifungal, Antioxidant, Antibacterial	Agoramoorthy et al. (2007)
4	9,12-Octadecadienoic acid (Z,Z)-, methyl ester	Linoleic acid methyl ester (fatty acid methyl ester)	Antibacterial	Lima et al. (2011Lima L.A.R. et al. In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A.St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Rev Soc Bras Med Trop. 2011;44:777-80.)
5	9-Octadecenoic acid (Z)-, methyl ester	Oleic acid methyl ester (fatty acid methyl ester)	Antimicrobial, Nematicidal	Chandrasekharan et al. (2008Chandrasekharan M., Kannathasan K., Venkatesalu V. Antimicrobial activity of fatty acid methyl esters of some members of chenopodiaceae. Z Naturforsch C. 2008;63:331-6.); Lima et al. (2011Lima L.A.R. et al. In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A.St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Rev Soc Bras Med Trop. 2011;44:777-80.)
6	1,2-Benzenedicarboxylic acid, mono(2ethylhexyl) ester	Aromatic dicarboxylic ester	Antimicrobial, Cytotoxicity, Antioxidant, Antiinflammatory, Antiviral	Govindappa et al. (2014Govindappa M. et al. Chemical composition of methanol extract of endophytic fungi, Alternaria sp. of Tebebuia argentea and their antimicrobial and antioxidant activity. Int J Biol Pharm Res. 2014;5:861-9.); Krishnan et al. (2014Krishnan K., Mani A., Jasmine S. Cytotoxic activity of bioactive compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester extracted from a marine derived Streptomyces sp. VITSJK8. Int J Mol Cell Med. 2014;3:246-54.)