Abstract
Poly(3-hydroxybutyrate) [P(3HB)] is a natural, biodegradable polyester and is considered an attractive substitute for the petrochemical polymers, because has advantage of being degraded in the soil within a few months by microorganisms. This work exploits three strategies to synthesize P(3HB) from of the Cupriavidus necator with glycerol as co-substrate: cultivation without glycerol, with addition of 20 g L–1 glycerol in the phase of polymer producing and addition of 20 g L–1 of glycerol at the start of cultivation, in order to evaluate its effect on cell growth and the synthesis of the polymer. The results showed that the addition of glycerol at the start of culture led to highest values of accumulation percentage the P(3HB) (64.12%). However, in the experiments without glycerol can be observed higher values toward biomass to substrate yield (0.17 g g–1). These parameters presented statistically significant differences in function of the cultivation strategy used.
Keywords:
biodegradable polymer; biosynthesis; glycerol; poly(hydroxyalcanoate)
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