Statistical comments on “Antiretroviral changes during the first year of therapy”

Dear editor,

We read with great interest a recent article by Bandeira et al.¹ entitled “Antiretroviral changes during the first year of therapy.” In this study, the authors investigated the first year of highly active antiretroviral therapy (HAART) in patients from a reference center on HIV/AIDS management in Fortaleza. They evaluated CD4 T lymphocyte (LTCD4) count and viral load (VL) over the course of the treatment (4 time-points of measurement including before treatment, 2-4 months, 5-8 months and 9-12 months). As stated in the methods section and chart 1 of the article, the authors used Student t-test (independent t-test) and Mann-Whitney test for comparison of LTCD4 count and VL between different time-points of measurement. Indeed, the authors investigated levels of the numerical variables in one sample of the patients in different times and assumed them as independent measurements. Since they used only one sample of the patients and measured numerical variables in them during different time-points, their measurement are dependent. Student t-test and Mann-Whitney test are used to compare the differences between the means of two independent (unrelated) groups, but the measurements of the mentioned study are not independent.^2-6 Therefore, after assessment of the normal distribution of the variables, due to dependence of measurements, they must use dependent t-test (paired t test) or Wilcoxon signed rank test for comparisons between different time-points of measurement.^4,7-10

Taken together, we believe that most of the statistical tests used in this study (including Student t-test and Mann-Whitney test) are inappropriate, and the authors’ valuable study could be better used as citable experimental evidence if analyzed with appropriate statistical tests (including dependent t-test or Wilcoxon signed rank test).

References

• ¹
  Bandeira ACPCS, Elias DBD, Cavalcante MG, Lima DGL, Távora LGF. Antiretroviral changes during the first year of therapy. Rev Assoc Med Bras. 2017; 63(7):606-12.
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  Gaddis ML. Statistical methodology: IV. Analysis of variance, analysis of covariance, and multivariate analysis of variance. Acad Emerg Med. 1998; 5(3):258-65.
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  Farrokhi M, Arjaki D. Statistical comments on "Cytokine and chemokine profiles in patients with neuromyelitis optica spectrum disorder". Neuroimmunomodulation. 2017; 24(2):120.
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  Farrokhi M, Masoudifar A, Peykanpour F. Interleukin 17 and 10 in relapsing remitting multiple sclerosis. J Neurol Sci. 2017; 378:63.
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  Farrokhi M, Shirian N. Statistical comments on "no seasonal variation in physical activity of Han Chinese living in Beijing". Int J Behav Nutr Phys Act. 2017; 14(1):151.
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  Farrokhi M, Peykanpour F. Statistical comments on "Salivary iron (Fe) ion levels, serum markers of anemia and caries activity in pregnant women". Rev Bras Ginecol Obstet. 2017; 39(10):583.
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  Farrokhi M. Reply to: Statistical support for Sema3A and multiple sclerosis. Gene. 2017; 631:52.
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  Farrokhi M, Peykanpour F. Vascular endothelial growth factor-loaded bioresorbable delivery system for pulp regeneration. J Endod. 2017; 43(9):1414.
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  Farrokhi M. Sema3A and multiple sclerosis. Gene. 2017; 615:41.
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  Farrokhi M, Amani-Beni A. Statistical comments on” Assessment of musculoskeletal strength and levels of fatigue during different phases of menstrual cycle in young adults”. J Clin Diagn Res. 2017; 11(7):CL01.

Publication Dates

• Publication in this collection
  Jan 2018

History

• Received
  25 Dec 2017
• Accepted
  6 Jan 2018