With the objective of testing in vitro regeneration through organogenesis from stem explants of three hybrid clones of Eucalyptus grandis x Eucalyptus urophylla, the effects of the growth regulators TDZ [1-phenil-3-(1, 2, 3-thiadiazol-5-il)urea], BAP (6-benzilaminopurine) and NAA (Naphthalene acetic acid) were evaluated. In general, a differentiated clone response in relation to intensity, texture, color and oxidation degree of calli was observed due to growth regulator treatments. The best results in callus formation were recorded for the treatments with the combination TDZ (0,5 mg L-1) and ANA (0,1 mg L-1), achieving 100% of callus formation from the stem explants. Compact node structures appeared mainly in the extremities of the stem explants, being those areas responsible for the regeneration of adventitious buds. The best regeneration response was obtained with 1,0 mg L-1 BAP.
microprogation; growth regulator.and in vitro propagation
