The aim of this work was to isolate native wood white-rot fungi from fungal fruit-bodies and eucalyptus wood fragments from different regions of Brazil and to test their potential for degrading dead stumps and roots in Eucalyptus plantings after harvest. Fungi isolates were obtained in a culture medium composed by Eucalyptus sawdust and agar. Among 292 isolates submitted to the Banvedamm test, 144 were classified as phenoloxidases producing isolates. Among nine C/N ratios tested, it was observed a tendency of occurring larger chip decay in the C/N ratios equal to 60 : 1, 200 : 1 and 300 : 1. Two decay assays were performed in wood chips of Eucalyptus saligna using the C/N ratio 60 : 1 in order to verify which isolates were able to cause larger reduction of dry matter. In the first assay, which was evaluated at 90 days of incubation, seven isolates that stood out in relation to Trametes versicolor were selected. The second assay evaluated the capacity of 46 isolates in promoting decay. The isolates selected in the first assay were among the 11 most efficient in the second assay. Based on DNA analysis, six isolates were identified as Pycnoporus sanguineus (3 isolates), Peniophora sp., Pestalotiopsis sp. e Ganoderma sp.
Microbiological decay of stumps; Eucalyptus; wood white rot fungi
