ELECTRICAL CONDUCTIVITY IN <i>Piptadenia moniliformis</i> Benth. SEED LOTS CLASSIFIED BY SIZE AND COLOR

Azeredo, Gilvaneide Alves; Paula, Rinaldo César de; Valeri, Sérgio Valiengo

doi:10.1590/0100-67622016000500009

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Articles • Rev. Árvore 40 (5) • Sep-Oct 2016 • https://doi.org/10.1590/0100-67622016000500009 copy

ELECTRICAL CONDUCTIVITY IN Piptadenia moniliformis Benth. SEED LOTS CLASSIFIED BY SIZE AND COLOR

CONDUTIVIDADE ELÉTRICA EM LOTES DE SEMENTES DE Piptadenia moniliformis Benth. CLASSIFICADAS POR TAMANHO E COLORAÇÃO

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Among the most widely used tests for evaluating seed vigor, electrical conductivity (EC) stands out as one of the most promising due to its speed and simplicity. This study aimed to verify if the EC test could be used to evaluate the physiological quality of seed lots of Piptadenia moniliformis Benth. A seed lot, which was classified by seed tegument color and size, was used based on seed retention in round-hole screens, sizes 11 and 15, constituting 10 sub-lots. Prior to germination and the EC test, the seeds were immersed in concentrated sulfuric acid for 20 minutes in order to overcome tegument dormancy. During the EC test, we soaked seeds in 75 mL of water at 25 ºC and tested eight soaking periods (2, 4, 6, 24, 48, 72, 96, and 120 h) and two seed quantities (25 and 50) per replicate. The EC test, regardless of the number of seeds or soaking period, was not adequate for evaluating the physiological quality of color- or size-classified seed lots from P. moniliformis Benth. .

Keywords:
Vigor; Forest seeds; Rapid Tests

Table 1 Water content (WC, %), germination (G, arcoseno(G/100)0.5), first counting (FC, arcoseno(FC/100)0.5) and index of germination speed (IGS) of different seed sub-lots of Piptadenia moniliformis Benth., classified by seed color and size.

Size (Sieves)	WC (%)	G	IGS	FC
	Light Seeds
S11	11	82 ab	9.8 a	66 ab
S12	12	78 ab	9.0 a	63 ab
S13	12	65 b	8.2 a	60 b
S14	13	87 a	10.7 a	76 a
S15	13	77 ab	9.6 a	72 ab
CV (%) =	-	9.62	20.93	11.02
	Dark Seeds
S11	11	73 a	8.4 a	62 ab
S12	11	78 a	9.8 a	70 ab
S13	13	71 a	8.7 a	59 b
S14	13	80 a	9.8 a	75 a
S15	13	76 a	9.9 a	73 ab
CV (%) =	-	10.23	22.80	10.49

Table 2 Summary of the analysis of variance for the electrical conductivity of Piptadenia moniliformis Benth. seeds, separated by seed coloration and size, using samples of 25 and 50 seeds soaked in 75 mL of distilled water for different time periods.

Sources of Variation	Mean Squares
Sources of Variation	25 seeds	50 seeds
Treatments	30404.65	23860.60
Size (T)	72858.98^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	57978.57^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
Color (C)	166762.19^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	6188.33^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
Period (P)	163286.15^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	163283.44^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
T X C	13138.23^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	13611.39^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
T XP	9247.65^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	7520.24^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
C X P	36998.81^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.	6854.96^ significant at 1% probability, by the F-test. significativo a 1% de probabilidade, pelo teste F.
RESIDUE	1593.43	594.500
Mean	166.43	149.67
CV (%) =	23.98	16.79

Table 3 Electrical conductivity of Piptadenia moniliformis Benth. seeds, classified by color and size, using samples of 25 and 50 seeds soaked in 75 mL of water at 25º C (averages from different periods of soaking).

Size	Light		Dark	Means
		25 seeds
S11	130.36 Ac		119.75 Ab	125.06
S12	146.29 Ac		124.73 Bb	135.51
S13	220.09 Aab		159.66 Ba	189.88
S14	239.09 Aa		159.56 Ba	199.33
S15	210.45 Ab		154.30 Ba	182.38
Means	189.26		143.60
		50 seeds
S11	114.47 Ac		107.28 Ac	110.87
S12	127.11 Ac		119.86 Ac	123.48
S13	152.38 Bb		190.11 Aa	171.24
S14	184.66 Aa		151.10 Bb	167.88
S15	191.73 Aa		158.03 Bb	174.88
Means	154.07		145.48

Table 4 Electrical conductivity of different sub-lots of Piptadenia moniliformis Benth. seeds using samples of 25 and 50 seeds soaked in 75 mL of distilled water at 25 ºC for different periods.

Size	Soaking periods (hours)
	2	4	6	24	48	72	96	120	Means
	Light seeds - 25 seeds
S11	75.45 a	105.53 a	107.34 a	133.43 a	139.61 a	148.92 c	157.54 b	175.06 c	130.36
S12	78.75 a	112.03 a	118.05 a	151.58 a	165.54 a	171.22 bc	178.24 b	195.10 c	146.31
S13	92.89 a	127.65 a	138.75 a	185.88 a	207.75 a	282.01 a	332.04 a	393.72 b	220.08
S14	101.89 a	123.60 a	125.75 a	155.63 a	185.08 a	254.94 ab	397.98 a	567.88 a	239.09
S15	105.80 a	122.83 a	125.30 a	140.29 a	158.38 a	206.05 abc	303.24 a	521.72 a	336.72
Means	90.95	118.32	123.03	153.36	171.27	212.62	273.20	370.69
CV (%) = 28
	Dark seeds - 25 seeds
S11	70.01 b	96.97 b	101.73 b	121.17 b	124.12 b	133.79 c	145.35 b	164.90 c	119.75
S12	69.29 b	97.80 b	101.12 b	127.30 ab	136.91 ab	143.76bc	154.34 b	167.07 c	124.69
S13	89.77 ab	110.45 ab	116.51 ab	151.46 ab	161.63 a	183.61 a	210.98 a	252.84 a	159.65
S14	112.70 a	133.41 a	136.67 a	161.18 a	161.03 a	171.83 ab	191.49 a	208.21 b	159.56
S15	110.73 a	127.28 ab	132.87 ab	157.46 a	159.92 a	167.19 abc	179.45 ab	199.51 bc	154.30
Means	90.50	113.18	117.78	143.71	148.72	160.03	176.32	198.50
CV (%) = 12
	Light seeds - 50 seeds
S11	68.70 a	90.37 a	99.20 a	118.37 a	121.83 a	127.24 c	135.10 c	154.97 c	114.47
S12	75.83 a	98.05 a	110.70 a	122.34 a	130.73 a	138.26 c	156.78 c	184.18 c	127.10
S13	79.36 a	94.61 a	102.45 a	138.46 a	148.66 a	166.89 bc	210.28 b	278.35 b	152.38
S14	85.15 a	88.28 a	94.01 a	118.33 a	132.64 a	195.35 ab	312.30 a	451.52 a	184.69
S15	81.81 a	95.49 a	101.91 a	126.56 a	161.37 a	218.60 a	312.28 a	435.83 a	191.73
Means	78.17	93.16	101.65	124.81	139.04	169.26	225.34	300.97
CV (%) = 14
	Dark seeds - 50 seeds
S11	60.25 a	73.84 a	82.61 a	104.06 a	110.26	b 122.73 c	138.93 d	165.72 c	107.30
S12	67.87 a	92.81 a	99.11 a	118.57 a	126.24 ab	130.53 c	145.20 cd	178.54 c	119.85
S13	90.87 a	99.75 a	108.12 a	144.35 a	175.12 a	227.23 a	298.42 a	376.99 a	190.10
S14	95.93 a	112.35 a	118.48 a	143.26 a	157.48 ab	171.04 bc	193.91 bc	216.36 bc	151.10
S15	91.69 a	104.38 a	115.45 a	152.16 a	172.13 a	187.36 ab	205.72 b	235.38 b	158.03
Means	81.32	96.62	104.75	132.48	148.24	167.77	196.43	234.59
CV (%) = 19

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