Black wattle (Acacia mearnsii de Wild.) is difficult to micropropagate due to the low ability of multiplication and development of shoots. Thus, the present study aimed at determining the influence of various cytokinins on axillary bud proliferation in nodal segments of A. mearnsii. Explants from in vitro germinated seedlings were inoculated on B5 (Gamborg et al., 1968) basal medium. BAP (6-benzylaminopurine), BA (6-benzyladenine), 2iP (gamma,gamma-dimethylallylamino-purine) and Kinetin (6-furfurylaminopurine) were tested at the concentrations 1 mgL-1, 2 mgL-1, and 3 mgL-1. A randomized block design, in factorial arrangement with 6 replications, and 5 plants per plot was used. The assessments were made after 30 days, by counting the elongated shoots and the presence of callus. The use of BAP at 2 mgL-1 promoted the highest rate of bud multiplication (3,5 shoots/explant).
vegetative propagation; plant regeneration; tissue culture
