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The Effect of Serial Culture and Storage on the Protective Potential of a Competitive Exclusion Preparation

Efeito do Cultivo Seriado e Estocagem Sobre o Potencial de Ação do Método de Exclusão Competitiva

The competitive exclusion method (EC) was used to protect day-old birds against colonisation of the intestinal tract by Salmonella enterica serovar Enteritidis (SE). The culture prepared in nutrient broth incubated at 37ºC/24 h inhibited SE growth in the caecal contents. The beneficial effect was also observed after serial passage of the feces culture for up to 14 times. The material obtained after 12 serial sub-cultures after storage for 28 days at 4-6ºC also kept its action on SE.

aerobic faecal culture; competitive exclusion; Salmonella Enteritidis


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